الفهرس 
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Abstract
Mesenchymal stem cells (MSCs) are multipotent adult cells that capable of self-renewal and multilineage differentiation. Mesenchymal stem cells have been suggested to have a potential therapeutic role in neurodegenerative diseases.
Multiple sclerosis is an auto-immune demyelinating disease that leaves the patients with disabilities. Conventional drug therapies for MS are not able to stop the degeneration of nerve tissue because current treatments focus in reducing the inflammatory lesions within the CNS but do not enhance endogenous myelin repair.
This study was conducted to examine the histological changes of cuprizone induced demyelination on white matter of mice brain, to explore the role of transplanted adipose derived mesenchymal stem cells in repair of the toxic model of cuprizone so that, discovering therapeutic strategies to replace myelin when complete endogenous remyelination is unsuccessful.
Cuprizone model of multiple sclerosis was introduced by feeding mice daily with cuprizone (bis-cyclohexanone oxaldihydrazone) mixed with standard chow (0.2%) for 6 weeks.
The present study was carried out on 36 adult female mice. 36 female mice were randomly divided into three groups, 12 animals each:
• group I (control group): The animals were fed with cuprizone-free normal diet.
• group II (demyelination group): The animals were fed daily with cuprizone for six weeks. By the end of the sixth week and cessation of cuprizone intake (day 0), they received a single intravenous injection of 500 µl of a complete culture medium into the tail vein (day 1).
• group III (transplanted group): The animals were fed daily with cuprizone-containing diet for 6 weeks followed by a single intravenous injection of about 1 × 106 AD-MSCs suspended in 500 µl of a complete culture medium into the tail vein (day 1).