الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 157 |  |  |
| | | from | 157 | | |
Abstract
the present study was conducted using an eco-friendly biotic and abiotic components for controlling most problematic soil borne and sclerotia-forming fungi Sclerotium rolfsii, Sclerotinia sclerotiorum and Sclerotium cepivorum. Effect of H2O2 towards T.harzianum (TrA1d) growth parameters. H2O2 significantly reduced radial growth, sporulation and mycelial dry weight even at 0.4 g/l and its inhibitory effects increased with increasing H2O2 concentration where highest inhibition percentage at 1.2 g/l H2O2. An obivious positive effect for H2O2+AA to enhance the antagonistic potentiality of TrA1dtowards S. rolfsii, Sclerotinia sclerotiorum and S. cepivorum.. Increasing H2O2 concentration enhanced antagonistic potentiality of TrA1d. At highest H2O2 concentration 1.2 g/l H2O2 antagonistic potentiality of TrA1d exhibited 98, 73 and 83 % inhibition in radial growth and 90,78 and 89% inhibition in sclerotia formation against S.rolfsii, Sclerotinia sclerotiorum and S. cepivorum ,respectively. Crude TrA1d culture filtrate has inhibitory effects towards radial growth and sclerotia formation against all three fungi tested. Heated CF showed significant inhibitory effects but lower than crude CF. Results exhibited protein changes for S. rolfsii and S. cepivorum but no changes recoreded with Sclerotinia sclerotiorum upon treated by AA and/or H2O2. A significant reduction of Sclerotinia sclerotiorum infectivity upon PAA application as seeds treatments or bean pod spraying. Seeds treatments with PAA significantly reduced root rot /stem blight severity from 93% in control to 49 and 30% when soil was drenched by 0.4 g/l AA +0.8 g/l H2O2 and 0.4 g/l AA+1.2 g/l H2O2, respectively.