الفهرس 
Scorpions
Materials and MethodsOnly 14 pages are availabe for public view
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Abstract
Rheumatoid arthritis (RA) is a systemic autoimmune inflammatory disease, the primary expression of which occurs in the synovial tissues especially joints. On the other hand, scorpion venoms are known as a source of peptidyl inhibitors of various types of potassium-channels which thus might play a potent treatment of autoimmune diseases including RA. Therefore, in this study the crude venom was collected from A. amoreuxi scorpions using electrical stimulation then processed and A. amoreuxi venom of 1/5 and 1/10 LD50 were chosen as subcutaneous treating doses to rats. RA in all animal groups was induced using a single subcutaneous injection of 0.1 ml of Complete Freund’s Adjuvant CFA the footpad of the right hind of 32 adult male albino Wistar rats paw except negative control group. Untreated arthritic group, Scorpion Venom (SV) low dose, SV high dose and Methotrexate treated groups were analyzed and compared using body measurements and behavioral Tests in addition ,physiological, histological and immunohistochemical tests. SV low dose, SV high dose and Methotrexate treated groups showed significant reduction in both paw swelling and pain perception score compared with non-treated arthritic group. Also, same groups showed increase in motility score parameter compared with non-treated arthritic group. Histological examinations showed significant regression of inflammatory process in SV low dose, SV high dose and Methotrexate treated groups comparing with non-treated arthritic group. Also, Immunohistochemical staining showed significant decrease in expression of inflammatory mediators in SV low dose, SV high dose and Methotrexate treated groups comparing with non-treated arthritic group.