الفهرس 
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Abstract
The present study was carried out to determine the effects of probiotics (Bacillus subtilis and B. amyloliquefaciens) administration in ration for 15 and 30 days on hematological, biochemical, immunological profiles and disease resistance of Clarias gariepinus to Pseudomonas aeruginosa with confirmation by histopathological examination.
This study was held on 160 Claris garpineous with an average body weight (141.96 ± 3.09 g), which were divided into four groups (40 fish/group). Each group was subdivided into two subgroups (A and B; each subgroup had two replicate of 10 fish): subgroup A was subjected to the experimental diet feeding for 15 days; and subgroup B was fed on experimental diet for 30 days. group one (G1) was fed on normal basal diet (D1). Groups 2, 3 and 4 (G2, G3 and G4) were fed on experimental diet D2 (supplemented with 0.5 g/kg of Biogen-s), D3 (supplemented with 1g/kg of Ecobiol Aqua) and D4 (supplemented with mixture of Biogen-s and Ecobiol Aqua), respectively.
At the end of 15 (subgroup A) and 30 days (subgroup B), the blood sample was collected for immunological and biochemical analysis. The fish after 15 days or 30 days of feeding was challenged with 0.5 ml of Pseudomonas aeurginosa (1x107) Intra peritoneal (I/P); this bacterial strain was previously been isolated from moribund fish and confirmed to be pathogenic through the project No 5589 at the department of Fish Diseases and Management. Fish was kept under observation for recording the clinical signs, mortality rate, and post-mortem lesions up to 15 days. Specimens from the liver and kidney were collected at the end of treatment trials for histopathological examination.