الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
Endodontic microsurgery is commonly used for treatment of teeth with persistent pathosis or where conventional retreatment methods are contraindicated. After root resection and retrograde cavity preparation, retro-filling material is used to fill the cavity. Biodentine is a commonly used material for this purpose. During the application of retro-filling material, blood contamination could happen which might affect the biological and mechanical properties of the used material.
The aim of this study was to evaluate the effect of blood contamination taking place during setting of Biodentine retro-filling material on its bond-strength and bioactive properties.
Forty extracted single rooted sound human anterior teeth were prepared and obturated. Roots were resected, retrograde cavities were ultrasonically created and Biodentine retro-filling was inserted. Teeth were divided into 2 groups (n=20) according to the liquid in which setting of Biodentine retro-filling took place. group I was allowed to set in deionized water as a control group. group II was allowed to set in blood. After complete setting, dentin discs 2 mm in thickness with the filling material in their core were obtained. These discs were randomly divided into subgroups according to the property under evaluation. Subgroups I-A and II-A for bond strength evaluation. While sub groups I-B and II-B for bioactivity evaluation.
Push-out bond strength testing was performed using universal testing machine while bioactivity testing was done using SEM for morphological analysis and EDX for elemental analysis and calculation of Ca/P ratios, as well as XRD for phase analysis. Results were tabulated and statistically analyzed.
The results showed that in this study, there was a statistically significant difference in the bond strength and bioactivity values for the blood-contaminated group in comparison to the non-contaminated control group, in which blood had a negative effect on the bond strength and bioactive properties of Biodentine retro-filling.
Conclusions
Under the circumstances of this study, it can be concluded that:
• Blood contamination during setting of Biodentine had a negative effect on its dislocation resistance.
• Blood contamination had a detrimental effect on Biodentine bioactivity.