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Abstract Strangles is the most important respiratory disease affected equine world wide causing economic losses as the disease course is long and need long recovery period with some complications which may ended with death of the animals .The disease affected the performance and growth rate of affected animals in addition to cost of treatment. This work was aimed to study some epidemiological and clinical forms of strangles, using PCR as the diagnostic method for confirmation the bacteria caused disease in addition to bacteriological examination also detected the most antimicrobials affected on the disease depends on the sensitivity test . As clinical forms of disease there were typical and atypical form, typical form was characterized by systemic reaction including increase of body temperature (40°C) ,congested mucus membrane, unilateral, bilateral mucopurulent nasal discharge, moist cough, extension of head and neck to relieve pain, inflamed and enlargement of sub mandibular L.N or abscessated L.N, and represented in 2015, 2016, 2017 as 33.33% , 25% , 39.5% respectively. While atypical form was characterized by by mild systemic reaction including mild increase of body temperature (39°C), mucopurulent nasal discharge, slight enlargement of L.N, and represented in 2015, 2016, 2017 as 66.67%, 75%, 60.5%.The incidence of strangles during period Jan2015 till April2017 as it was higher in January 2015 was 6.27 % and in March ,April 2017 was 13.59% ,11.23% respectively with total incidence 45.77 %.The seasonal incidence of strangles showed that the highest incidence rate in winter with percent 20% and in spring with percent 14.5 %.All ages of horse contracted disease but the most ages affected with strangles was in range six months till three years as showed that 58 cases and 29 cases less than six months also 10 cases from three years to five years. As using PCR for detection of Se-M gene that was specific for S.equi sub species equi which cause disease this study revealed that there was three positive isolate from eight isolate which represented 37.5%. Streptococcus equi isolates were tested for their sensitivity to different antimicrobials agents and the study revealed that S.equi was sensitive to Ceftriaxone , Cefotaxime (77%), Cefquinome (54%), and intermediate in Ciprofloxacin (92%) , Amoxicillin calvulanic acid (70%)and also resistant to Erythromycin (85%), Sulphonamide (77%), Vancomycin (70%), florfenicol (70%). |