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Abstract This study aims to evaluate screening different native plant growth promoting bacteria (PGPB) conjugated with Arbuscular mycorrhiza (AM) inocula to induced resistance in grapes against grape fan leaf virus( GFLV) infected grape. Obtained results reveled that more them 30 native bacterial isolates of Bacillus, Pseudomonas, Serratia were obtained from rhizosphere of grape were characterized depending on their morphological, cultural characters .isolates were selected according to their biochemical properties. The most effective strains were selected identified according to Hgene based on 16s RNA. Obtained results revealed that they are Bacillus megaterium, Pseudomonas flourescens, Serratia marcescens. Results clearly indicated that plant growth promoting microorganism (PGPM) inoculation in soil improves plant growth in the second season of cultivation (2015/2016) compared with first season (2014/2015(. However, selected strains of PGPM conjugated with AM improve quality of GFLV infected grape plants via increased plant growth parameters. PGPM induced acquire resistance in plant against GFLV; it was found that, significant increase of proline and salicylic acid (SA) contents in GFLV infected grape leaves compared with healthy ones. Chlorophyll a; b and carotenoids were significantly decreased while inoculated PGPM in soil showed significant increase compared with healthy control ones. Expressed proteins and resistance enzymes peroxidase (POD) and polyphenol oxidase (PPO) of antiviral proteins were significant increase in PGPM application of GFLV infected grape growth compared to control treatment. The current study recommended that the combination between AM and effective mixed bacterial strains of PGPR soil inoculation improved quality of GFLV infected grape plant under Greenhouse conditions. Keywords: Grape fan leaf virus (GFLV), Plant Growth Promoting Bacteria (PGPB), Arbuscular mycorrhiza (AM), Induced Systemic Resistance (ISR) |