الفهرس 
AcknowledgementOnly 14 pages are availabe for public view
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Abstract
Objectives: This in vitro study evaluated the effect of Essential oils
(EOs), Essential oils with sodium fluoride (EOF) and Chlorhexidine
(CHX) mouth washes on viability and apoptosis of fibroblast cell line.
Material and Methods: Baby hamster kidney -21 (BHK-21) fibroblast
cell line was cultured and incubated in Eagle’s Minimum Essential
Medium (EMEM) containing, EOs, EOF and CHX with different doses
and various exposure times. Control cells received an equal volume of
EMEM without adding mouth rinses. Cell viability and apoptosis were
evaluated using MTT assay and RT-PCR. Results: The EOs, EOF and
CHX mouthwashes inhibited fibroblast cell viability in a dose and time
dependant manner. Conclusions: CHX mouthwash was the most
cytotoxic to fibroblasts as compared to EOs and EOF. Moreover, adding
fluoride to EOs in low concentrations didn`t worsen the adverse effects
of EOs on cell viability and apoptosis induction.