الفهرس 
FIRE RELATED DEATHSOnly 14 pages are availabe for public view
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Abstract
Introduction: Identification of burnt bodies is a great challenge as the ordinary methods of identification is impossible, so DNA is the only available method of identification. Teeth can be used as a source of DNA in charred bodies as they are largely protected from environmental and physical conditions.
Aim: This study was designed to assess integrity of the DNA recovered from isolated human teeth after being exposed to high grades of temperature as a tool for forensic identification.
Material and Method:
Extracted teeth were subjected to the following temperatures: 100ºC, 300ºC and 500ºC, teeth Pulp from teeth were retrieved by dental file, followed by
extraction of DNA with Quiagen kit followed by PCR for amplification of the tested loci (D21S11, TH01, CSF1PO, D18S51, D7S820, D13S317), then gel electrophoresis was done to visualize DNA bands.
Results : No significant difference was found from control samples on exposure to 100ºC, on exposure to 300ºC there was significant difference from control in all tested loci except in TH01 while on exposure to 500ºC there was complete loss of DNA.
Conclusions: Dental pulp is a good source of DNA. Exposure to high grades temperatures degrades DNA from dental pulp and at 500ºC genomic DNA is completely lost