الفهرس 
Cardiovascular system
Oxidative stress and inflammationOnly 14 pages are availabe for public view
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Abstract
Hawthorn (Crataegus Oxyacantha) is one of the most important traditional medical plants which have been used in treating various cardiovascular diseases (CVDs) such as myocardial infarction (MI).MI is one of the most frequent CVDs causes of death all over the world. This study had been designed to investigate the influence of ethanolic hawthorn leaves extract (EHLE) administration at 200 mg/ kg body weight/day on isoproterenol (ISO)-induced MI by measuring different biological and biochemical parameters in rats. In the present study, 50 adult male white albino rats Sprague-Dawely strain weighing185 ± 15g were used and divided into five groups comprising of ten rats in each group. group I: served as healthy untreated rats. group II: healthy rats treated with EHLE at tested dose by gastric tube three times weekly. group III: (MI group): rats were injected with ISO subcutaneously at dose of 85mg/kg body weight/day on two consecutive days with 24 hours intervals. group IV: MI rats treated with EHLE. group V: rats treated with EHLE pre and post MI induction. The results of EHLE bioactive components analysis revealed that each one gram of the tested extract contains 70.048 ± 0.5254 mg as gallic acid equivalent (GAE) for total phenols, and 154.417± 0.7406 mg as quercetin equivalent (QE) for total flavonoids, as well as high free radical scavenging activity, where 0.05 ml of the tested EHLE scavenged 5 ml of diphenyl-2-picryl hydrazyl (DPPH) radical by 89.55 ± 0.5683%, also flavonoids and phenolic compounds were analyzed by HPLC method. The biological trail showed significant (P≤0.05) reduction in rats’ body weight change, feed intake and feed efficiency ratio (FER) and increment in both feed conversion ratio (FCR) and relative heart weight in MI group when compared with healthy untreated rat group. ISO induced significant (P≤0.05) increment in serum cardiac enzyme activities of AST, LDH, CK, and CK- MB as well as serum specific MI biomarkers of cTnI and Gal-3 levels and oxidative stress markers of NO and MDA, in association with a significant reduction (P≤0.05) in cardiac enzymatic and non-enzymatic anti-oxidant system. While, EHLE administration either post or pre and post ISO injection showed a significant improvement in all biological and biochemical parameters, confirming its cardio-protective effects. These results were confirmed by the histopathological examination of the heart tissues in different rat groups.