الفهرس 
Oral mucosaOnly 14 pages are availabe for public view
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Abstract
Oral ulcer, induced oral ulcer,formocresol, platelet rich plasma, mesenchymal stem cells, bone marrow stem cell, oral wound healing.Introduction: Oral ulceration is one of the most common debilitating and widespread condition that affects the oral cavity. The dynamic process of wound healing aims to replace devitalized and missing cellular structures and tissue layers and restore tissue integrity and function.Regenerative medicine concept is emerging as an alternative to conventional line of treatment and as a potential clinical application in wound healing. Platelet rich plasma(PRP)and mesenchymal stem cells represent a biological pool of wide range of growth factors and cytokines that are essential for optimum wound healing.Objectives and aim of the study: The aim of this study was to evaluate the therapeutic potentiality of locally injected bone marrow mesenchymal stem cells(BMSCs)versus PRP on healing of induced cheek ulcers in albino rats. This was achieved by both quantitative and qualitative methods.Materials and methods: An ulcer was induced in the buccal mucosa of rats(n=60) using 5mm biopsy punch and then a cotton swap soaked with formocresol was applied to the wound for 60 sec. The ulcers were left untreated in the control group or treated with intralesional injection of PRP in the PRP group or treated with isolated cultured BMSCs (1X106 cells) in the stem cell group. The results were evaluated clinically,histologically and immunohistochemically at day 3,5,7 and 10 after ulcer induction. Clinical evaluation was done using photographs,then the ulcers areas were measured by imageJ computer program. Histological tissue sections were prepared and stained with haematoxylin and eosin for general examination and with trichrome for collagen fibers and blood vessels examination. In addition, the sections were stained immunohistochemically using anti-PCNA antibody to measure the proliferative rate of the healing tissues.