الفهرس 
Bioprospecting
Isolation, Identification and ExtractionOnly 14 pages are availabe for public view
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Abstract
Red sea was recognized as a rich source of microbial diversity
with unique metabolites. Marine fungi are known to produce a great
variety of secondary metabolites with unique chemical structures that
have appealing bioactivities.
The present study was aimed to screen for different biological
activities in Sponge and Soft corals associated fungi of the Red Sea
coast at Hurghada and evaluate the effect of media composition on
the production of active secondary metabolite.
In this context, four marine sponge and coral associated fungi
[Aspergillus sydowii SP52-EGY, Aspergillus tamarii SP73-EGY,
Aspergillus sp. 2C1-EGY and Aspergillus ungusSPMD-EGY] were
isolated, identified and registered in Gene Bank. Applying the
OSMAC (One Strain Many Compounds) technique in this study, the
four isolated fungi were grown on four different media (A,B,C &D)
under shake and static conditions and the different biological
activities (antimicrobial, antioxidant, enzyme inhibitors and
anticancer activity) of the crude ethyl acetate extracts of secondary
metabolites were evaluated. The extracts of the four fungi
demonstrated variable biological activity in-vitro assays.
The secondary metabolite extracts of the four fungi were tested
against a panel of three cancer cell lines, HCT-116, HepG2 and
MCF-7 and the results revealed significant cytotoxic activity of the
extracts.
Extracts of the four sponge and coral associated fungi; which showed
high cytotoxic activity in the monolayer cell culture model (2D),were selected for further investigation in the three-D multicellular
spheroid (MCS) model (3D).Two active extracts in the 2D model (A
St F and D St F) showed significant cytotoxic activity on the (3D
MCS) for the fungus A. SPMD. The chemical composition of both
extracts were analyzed by GC/MS spectroscopy and showed totally
different chemical composition.
It was concluded that secondary metabolites of sponge and soft corals
associated fungi of the Red Sea coast considered as potential
producers of bioactive compounds that have antimicrobial,
antioxidant and anticancer activities.