الفهرس 
BiopolymersOnly 14 pages are availabe for public view
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Abstract
Due to the wide and inappropriate use of the antibiotics, the
continuous development of resistant strains of bacteria to the most of
common antibiotics has become a vital problem in public health;
leading to strong stimulant to continuity developing new and effective
antimicrobial agents. Nanotechnology considers as a magic tool to
explore and treat the difficult problems of medical sciences. The
confluence of nanotechnology and microbiology solves several
biomedical problems, and also can revolutionize the health and
medicine fields.
According to recent searches on antibacterial activity of both
chitosan (Ch) and Multiwalled carbon nanotubes (MWCNT), this
study deals with developing nanocomposites of both nanoparticles for
examining their antibacterial effects on pathogenic Gram positive and
Gram negative bacteria. The ability of the optimum nanocomposite in
removal of target heavy metals from aqueous solutions using two
different environmental wastewaters samples had also been studied.
The nanocomposite chitosan-multiwalled carbon nanotubes
(Ch-MWCNT) was synthesized by the process of sonication for 20
min. and examined for their antibacterial activity.Seven different
concentrations of MWCNT have been used in the preparation of
Ch-MWCNT (from 1 to 100 mg). The prepared nanocomposites with
different MWCNT concentrations were characterized using TEM and
FTIR. TEM results showed that the morphology of MWCNTs were
obtained in the form of small tubes in length as well as the modified MWCNT has a good distribution over the chitosan polymer
surface.While FTIR show that the presence of the C=C absorption at
the wave number 2344 cm-1which confirm the successful
incorporation between chitosan and MWCNT. For antibacterial
activity estimation, by using the disc diffusion technique towards
Gram positive and Gram negative bacteria. The composite Ch-
MWCNT showed higher antimicrobial activity against both Gram
negative and Gram positive bacteria with increasing MWCNT
concentration till ratio 1:2. The nanocomposites are highly
differentiable at the low concentration; 1% concentration of the
multifunctional nanocomposite is very effective against the tested
microbes. Minimal inhibitory concentrations (MIC) by using the
serial dilution technique of Ch-MWCNT (100:50 Ch-MWCNT)
against Gram negative and Gram positive pathogenic bacteria
between 0.5 to 0.0625 μg/mL, and the growth inhibition effect has
been observed in a concentration dependent species. There was
further investigated needed to remove heavy metal ions and
pathogenic microbial contaminants from industrial wastewater. The
nanocomposite also showed high efficiency for the removal of heavy
metal ions (Pb+2, Cu+2, Zn+2 and Cd+) from the industrial wastewater.
Key words: CNT, Chitosan, Nanocomposites, MIC, Removal of
Heavy metals.