الفهرس 
Insulin resistanceOnly 14 pages are availabe for public view
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Abstract
Patients and Methods
This study included 90 age and sex matched non diabetic patients, 30 un controlled hypothyroid, 30 uncontrolled hyperthyroid patients and 30 healthy euthyroid individuals as a control group. This study was conducted atFayoum University Hospital from the month of february to the month of june during 2015 .
-All subject were subjected to:
A- Medical history and clinical examination. Including vital statistics
( body weight , height , BMI and waist circumference ) were done and recorded.
B-Laboratory tests including
- Data of thyroid stimulating hormone levels were collected from patientś files
-Fastingblood sample (8 hours fasting) was collected on plain vacuotainers for: .
. Insulin level .Glucose level.
-Fasting glucose was performed immediately after sample collected
( Flexor).
-Fasting insulin level was done by ELISA technique (sandwiched) using Bioteck( USA).
- Serum was sparated and stored at -80ºc until analysis was done for fasting insulin.
- Test Principle:
The insulin quantitative test kit is based on a solid phase enzyme-linked immunosorbent assay. The assay system utilizes one anti- insulin antibody for solid phase (microtiterwees) immobilization and another anti-insulin antibody in the antibody-enzyme (horseradish peroxidase) conjugate solution. The standards and test specimen (serum) are added to the insulin antibody coated microtiter wells. Then anti-insulin antibody labeled with horse radish preroxidase (conjugate) is added. If human insulin is present in the specimen, it will combine with the antibody on the well and the enzyme conjugate resulting in the insulin molecules being sandwiched between the solid phase and the enzyme –linked antibodies. After a 1 hour incubation at room temperature, the wells are washed with water to remove unbound labeled antibodies. A solution of TMB is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is stopped with the addition of stop solution. The color is changed to yellow and measured spectrophotometrically at 450 nm. The concentration of insulin is directly proportional to the color intensity of the test sample ( Eastham, 1985).
Inclusion criteria
Adult male or female non diabetic Patients who were diagnosed clinically and by laboratory investigations and were receiving treatment for hypothyroidism or hyperthyroidism . The control group were investigated to exclude thyroid diseases , diabetes or prediabetes .
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