الفهرس 
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Abstract
Summary
Stromal cell derived factor -1 (SDF-1) is a peptide chemokine initially identified in bone marrow derived stromal cells and is also nominated as CXC motif ligand 12 (CXCL12).
SDF-1 is a small 68 amino acids, 8 kDa chemokine that has been identified as the ligand for cell surface CXC - chemokine receptor 4 (CXCR4) and receptor 7 (CXCR7).
Stromal cell derived factor -1 / CXC motif ligand 12 (SDF-1 / CXCL12) is a chemokine initially know to be expressed by bone marrow derived stromal cells and then recognized to be expressed in stromal tissues in multiple organ.
The gene encoding SDF-1 is located on the human chromosome 10 q11.1. Guanine to adenine (G to A) transition at position 801 of the 3’ untranslated region has been found to result in SDF-1β G801A gene polymorphism.
SDF-1β G801A gene polymorphism has been indicated to be associated with diabetes as well as other immune related diseases including AIDS and cancer.
In this study, our goal was to investigate SDF-1β G801A gene polymorphism in a sample of Egyptian population with T2DM as regards incidence and glycemic control.
The current study included 50 patients with type 2 diabetes mellitus their mean age (50.32 ± 10.82years); 15 males and 35 females. Fifty apparently healthy volunteers their mean age (50.80 ±11.88 years); 24 males and 26 females were included representing the control group.
Patients and control groups were subjected to:
. Full history taking*
Clinical examination. *
* Laboratory investigations including:
1) Routine laboratory investigations:
Fasting blood glucose and 2 hour post prandial (2h PP).
Lipid profile (cholesterol, triglycerides, LDL-C, HDL-C).
Glycated hemoglobin (HbA1c).[
Serum creatinine.
2) Genetic analysis:
Genomic DNA extraction and analysis for SDF-1β G801 gene polymorphism using polymerase chain reaction followed by restriction fragment length polymorphism (PCR-RFLP) method.
In our study, we found that no statistically significant difference was found between the diabetic group and control group as regards their genotypes (GG, AG, AA) (p = 0.084) and alleles (G, A) (p = 0.891).
In the present study, we found that the GG/AG genotypes showed higher frequency among diabetic patients than control group while AA genotype showed lower frequency in diabetic patients than control group but it didn’t reach statistical significance.
In the present study, we found that homozygous GG genotype and G allele showed statistically significant higher frequency among patients with HbA1c ≥ 8 % (p= 0.001) and (p= 0.018), respectively.