الفهرس | Only 14 pages are availabe for public view |
Abstract The present study was interested with the phytochemical and biological studies of some Egyptian medicinal plants as a source of natural products. This study was done on four plants (Tamarix aphylla, Brassica nigra, Thymelaea hrisuta and Euphorbia retusa). The anti-inflammatory properties of all the extracts, fractions and pure compounds separated from these plants were checked for their bioactive products. The present research work aims at:- 1- Screening the biological activities of the extract(s) and fraction(s) of selected medicinal plants. 2- Isolation and purification of the compound(s) from the plant extracts using chromatographic techniques. 3- Structure elucidation of isolated metabolites via spectroscopic techniques. 4- Determination of the bioactive compound(s) using bioassay guided isolation procedures. The present thesis is divided into: Chapter 1: Introduction It is including a brief introduction about XII 1- The importance of natural products in drug discovery. 2- Ethnopharmacology and potency of Egyptian medicinal plants. 3- Anti-inflammatory agents and inflammation. 4- Summary of literature survey done on the selected plants. Chapter 2: Materials and methods This section includes the following: 1- It describes the collection and identification of selected plants. 2- It describes the apparatus and methods used for extraction, isolation, purification and identification of the isolated compounds. 3- It describes the methods followed in the immunomodulatory activities (anti-inflammatory) examination of extracts and pure compounds. Chapter 3: Results and discussion Phytochemical investigation of Tamarix aphylla aerial parts leads to isolation of four compounds [3,5-Dihydroxy-7,4’-dimethoxyflavone(1); Methyl 4-O-methylgallate(2); 3,4’,5,7-Tetrahydroxyflavone (3); and Trans-3-Hydroxy-4-methoxycinnamic acid (4)]. The structures elucidation has been carried out using X-ray, UV, IR, one and two dimensional NMR, and high-resolution mass spectrometry followed by comparison with spectroscopic data of closely related structures. |