الفهرس 
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Abstract
Aim: This study aimed to evaluate the expression of p53 gene following treatment of induced oral cancer, in hamster buccal pouch (HBP), with thymoquinone (TQ) loaded 0.001 mg/kg. body wt on gold nanoparticles (GNPs).
Material and Methods: The study was done on archival paraffin blocks to study the effect of different TQ preparations on the chemically-induced squamous cell carcinoma(SCC) of HBP. The chemical carcinogen0.5% solution of 7,12 dimethyl benz-(a)-anthracene(DMBA), dissolved in heavy mineral oil, was topically applied toHBP 3 times /week for 14 weeks. The paraffin blocks represented left and right buccal pouches from 55 male Syrian golden hamsters, weighed 90-120 grams. They were divided into 4 major groups: group A (control groups): A1 (20 animals) served as negative control group (did not receive any treatment), and A2 (5 animals) served as positive control (painted with DMBA for 14 weeks). group B: (30 animals) as the experimental groups(painted with DMBA 3 times/week for 14 weeks) and then divided into group B1:5 animals were sacrificed after three weeks of i.p injection of TQ (0.001mg/Kg body weight), group B2: 5 animals were sacrificed after six weeks of i.p injection of TQ (0.001mg/Kg body weight), group B3: 5 animals were sacrificed after three weeks of i.p injection of GNPs, group B4: 5 animals were sacrificed after six weeks of i.p injection of GNPs ,group B5:5 animals were sacrificed after three weeks of i.p injection of (0.001mg/Kg body weight)TQ-GNPs and group B6: 5 animals were sacrificed after six weeks of i.p injection of (0.001mg/Kg body weight)TQ-GNPs. from each paraffin block an H&E stain was performed to confirm the histopathological changes and immunohistochemical stain for p53.
Results: Groups A1 (negative control)showed negative/mild p53 immune reactivity (score +0), while group A2 (positive control group) (DMBA for 14