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العنوان
Rapid detection of Salmonella species using molecular and nanotechnology techniques /
المؤلف
Aleslamboly, Yosra Samy.
هيئة الاعداد
باحث / يســرا سامي الاسلامبولي
مشرف / محمد كمـال رفاعـى
مشرف / محمود درديري الحريري
مشرف / محمد احمدالشاطر
الموضوع
Poultry products. Salmonella enteritidis. Salmonella typhimurium.
تاريخ النشر
2018.
عدد الصفحات
133 P. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
البيطري
تاريخ الإجازة
1/1/2018
مكان الإجازة
جامعة القاهرة - كلية الطب البيطري - Microbiology
الفهرس
Only 14 pages are availabe for public view

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Abstract

Bacteriological examination of 400 samples (200 poultry products and 200 meat products) revealed the isolation of 26 Salmonella belonging to 8 different Salmonella serovars. The most common serovars were Salmonella Typhimurium (8 isolates), Salmonella Enteritidis (7 isolates), Salmonella Kentucky (2 isolates) and Salmonella Virchow (2 isolates). Other serovars typed were Salmonella Heidelberg (4), Salmonella Haifa (1), Salmonella Anatum (1), Salmonella Farsta (1). All isolates of Salmonella Enteritidis and Salmonella Typhimurium were examined for the virulence genes spvA and fliC genes respectively. Molecular biology techniques Direct PCR as well as non-functional gold nanoparticles probe were used for direct detection of two serovars (S.Typhimurium and S.Enteritidis) in food samples.