الفهرس 
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Abstract
The current study was conducted in two consecutive years, 2010-2011. The study sites were selected to be inhabited by five nitrogen-fixing legumes namely: Melilotus indicus, Medicago polymorpha, Trifolium resupinatum, Trigonella hamosa and Vicia sativa.
The sites were representative of four Egyptian habitats of plants as follows:
a) Around the city of Assiut to represent the Nile region.
b) Around the city of Al-Kharga to represent the Oases region.
c) Burg El Arab west of Alexandria to represent the Mediterranean region.
d) The site of Saint Katherine in South Sinai to represent the Sinai region.
At each site, the following items were carried out:
1-Three soil samples were collected from depth up to 50 cm and transferred to the laboratory for physical and chemical analysis.
2- The nodulated plants of each region were assessed for the nodule number and fresh mass. Rhizobial strains from the nodule were isolated and identified.
3- Three individuals of each leguminous species were collected (as replicates) and after processing them and separating the roots from the shoots were immersed in liquid nitrogen and transported to the laboratory for the necessary analysis.