الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
Epigenetic studies have focused on understanding how histone proteins undergo posttranslational modifications that influence the architecture of chromatin. The fact that acetylation is a key component in the regulation of gene expression has stimulated the study of HDACs in relation to the aberrant gene expression often observed in cancer. The aim of this work was to investigate the activity of HDAC enzyme in tumor cell model Solid Ehrlich carcinoma under the effect of the anticancer drug methotrexate (MTX) in comparison with sulforaphane (SNF) extracted from cabbage leaves. To achieve the aim of this work cabbage leaf powder contains the SFN precursor glucoraphanin was autolysed in acidic pH followed by extraction with dichloromethane that finally evaporated and the remaining residue was dissolved in the acetonitrile solvent which is selective solvent for butanes such as SFN. Then extract was purified using silica gel. In this study, a sixty albino female mice were randomly equally divided into six groups: group I (Negative control group): This group included 10 untreated mice. The remaining mice were subjected to Ehrlich tumor cells which were implanted subcutaneously into the right thigh of the hind limb of mice and divided as follow: group II: Tumor bearing group “Positive control group”. group III: MTX treated group. group IV: SFN treated group before tumor implantation. group V: SFN treated group after tumor implantation. group VI: MTX and SFN treated group. At the end of experiment all mice were subjected to the following tests. Measurement of tumor weight and volume. Extraction of Tumor Nuclear Protein and determination of Histone Deacetylase (HDAC) Activity. Assessment of DNA Damage in the tumor nuclear extracts. Determination of Lipid Peroxide “Malondialdhyde” in the tumor and liver homogenates. Determination of total Antioxidant Capacity in mice sera and tissue homogenates “tumor and liver”. Quantitative Determination of serum AlT, AST, And Cr. Histological analysis of tumor tissues.