الفهرس | Only 14 pages are availabe for public view |
Abstract Fluorescence spectroscopy has many advantages making it an appealing choice for low concentrations samples analysis. Fluorometry may achieve limits of detection several orders of magnitude lower than those of most other techniques. This is known as the fluorescence advantage .fluorescence measurements are rapid and inexpensive, Because of the low detection limits, so fluorescence is widely used for quantification of trace constituents of biological and environmental samples. The study is divided into four chapters: Chapter one Introduction and literature review: This Chapter includes a general introduction on fluorescence spectroscopy and its advantage in modern analytical techniques and its theory about sensing and different types of fluorescent probes. A literature review on the measurement of enzymes such as glucose oxidase and 3-nitrotyrosine using different method of detection. Recent references related to research has been used. Summary Chapter two Describes a highly selective and sensitive spectrofluorimetric method for the assessment of 3-nitrotyrosine (3NT) in Serum Samples based on the quenching of luminescence intensity of the Optical sensor Pd-complex. It was found that there is energy transfer between photo probe and 3-nitrotyrosine in addition to measurement of photo probe emission at exication wave length (λEX =350 nm) under different condition of solvent and pH. The optimum conditions for measurement are DMF as solvent and pH=10.5 photo probe has been used for 3- nitrotyrosine concentrations by quenching of the photo probe(Pd-complex ) band at wave length of emission (λem=425 nm) after adding different concentration of 3-nitrotyrosine fluorescence intensity under optimum condition and making linear relation between fluorescence intensity and 1/different concentration and making wide linear working range (1.42 x 10−9 to 3.8 ×10−6 mol L-1 ) [3NT] with a correlation coefficient of 0.998 and a detection limit of 2.98 × 10−9 mol L-1. Thus, the use of the photo probe by measuring and estimating 3-nitrotyrosine in different samples of serum to the many of liver disease patients. Summary Chapter three Describes a highly selective and sensitive spectrofluorimetric method for the assessment of glucose oxidase [GO] in Serum Samples based on the quenching of luminescence intensity of the Optical sensor Pd-comlex. It was found that there is energy transfer between photo probe and H2O2 (which result from react β-D glucose + H2O + O2 in presence glucose oxidase enzyme) in addition to measurement of photo probe emission at exication wave length (λex =450 nm) under different condition of solvent and pH. The optimum conditions for measurement are acetonitrile as solvent and pH=9.5. photo probe has been used for GO concentrations by quenching of the photo probe band at wave length of emission (λem=530 nm) after adding different concentration of uric H2O2 fluorescence intensity under optimum condition and making linear relation between fluorescence intensity and 1/different concentration and making wide linear working range (1.0 × 10−9 to 5.0 ×10−6 mol L−1 (r = 0.998). And The detection limit (S:N = 3) (LOD) is 2.8 x 10-10mol L−1 .. Thus, the use of the photo probe in the enzyme glucose oxidase activity estimate by measuring and estimating H2O2 in different samples of serum to the many diabetes patients. Summary Chapter four The efficiency of excited-state interaction between Eu3+ doped in sol- gel matrix and the industrial product ciprofloxacin of (CFX) has been studied in different solvent and pHs. A high luminescence intensity peak at 617 nm of europiumciprofloxacin complex at λex=365 nm in acetonitrile was obtained. The photophysical properties of the red emissive Eu3+ complex doped in sol-gel matrix have been elucidated, the europium was used as optical sensor for the assessment of ciprofloxacin in the pharmaceutical tablets and serum samples at pH 8.0 and λex = 365 nm with a concentration range of 5.0 ×10−9 - 1.0 ×10−6 m ccxol L−1 for ciprofloxacin, correlation coefficient of 0.99 and detection limit of 1.65 ×10−9 mol L−1. |