الفهرس 
ContentsOnly 14 pages are availabe for public view
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Abstract
Nephrotic syndrome is a disorder of the kidneys that results from increased permeability of the glomerular filtration barrier. It is characterized by 4 major clinical characteristics that are used in establishing the diagnosis: proteinuria, hypoalbuminemia, edema, and hyperlipidemia. Edema is one of the classic clinical features of childhood nephrotic syndrome (NS) and one of the principal reasons for admission to the hospital. The underlying mechanisms of edema formation remain a subject of ongoing investigation. The role of ANP in the pathogenesis of nephrotic syndrome is uncertain. In experimental studies, increased ANP and blunted response to exogenous ANP have been demonstrated. In clinical trials ANP has been found to be elevated, normal or diminished in nephrotic syndrome. The aim of this study is to investigate the role of atrial natriuretic peptide gene polymorphism in the development of edema in childhood nephrotic syndrome and its response to therapy.Sixty-three children patients with active nephrotic syndrome (NS) presented with sever generalized edema and fulfilling the inclusion and exclusion criteria for the diagnosis of nephrotic syndrome were included in this study. Patients were recruited consecutively from Mansoura University Children’s Hospital from August 2014 to August 2015. All participants were subjected to: 1.careful history taking, 2. full clinical examination. 3. Routine laboratory investigations (serum albumin, Creatinine, hematocrit, urine analysis). 4.Molecular investigations include:DNA extraction and genotyping of ANP gene polymorphisms. ANP gene polymorphism (A2843G) may not play a role in edema formation in active nephrotic syndrome and cannot alone explain the cause of hypovolemic and non- hypovolemic state in those patients. Meanwhile the response of edema in those patients cannot be explained by ANP gene polymorphism (A2843G).