الفهرس 
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Abstract
One hundred sixteen quarters were collected aseptically from apparently healthy udders of twenty –nine cows in a dairy farm in Beni- suef city, Egyptian a sterile plastic test tube with tighten fitting caps which labeled with permanent marker before sampling. Samples were properly packed, stored in an ice box (at 4oC) and transferred to the laboratory with a minimum of delay to be examined chemically and microbiologically. Firstly each sample was detected for the incidence of mastitis by CMT then each sample was mixed thoroughly before being divided into two parts. The first was transferred to the lab of animal reproduction research institute, Giza for chemical examination and numbering of somatic cell count with minimum of delay, while the second one was used for microbiological examination.
The result of CMT and bacteriological examination revealed that 89 (76.72%) of the examined cow’s milk samples were negative and 27(23.28%) were positive, the results of yeasts and molds showed that 105(90.52%) of the examined cow’s milk samples were negative and 11(9.48%) were positive samples. Among the CMT positive samples, the incidences were recorded in CMT (+1),(+2) and (+3) as (40.74%),(59.26%) and (0.00%), respectively, while the incidences of CMT positive samples recorded 9(31.03%), 9(31.03%), 5(17.24%) and 4(13.79%) in fore left (FL), fore right (FR), hind left (HL) and hind right (HR),respectively.
The incidences of single infection were 2(7.41%), 2(7.41%), 5(18.52) and 3(11.11%) for FL, FR, HL and HR, respectively but the incidences of double infection were 4(14.81%), 4(14.81%), 0(0.00) and 1(3.70%) for FL, FR, HL and HR, respectively while the incidences of triple infection was 3(11.11%),2(7.41%) , 0(0.00%) and 0(0.00) for FL, FR, HL and HR, respectively and the incidences of tetra infection were 1(3.70) for FR and 0(0.00) for FL, HL and HR. The incidences of single and mixed infection were 44.44% and 55.56% in positive cows, milk samples for subclinical mastitis, respectively.
The isolated micro-organisms from the examined milk samples were 14.28, 12.24, 10.2, 40.82 and 22.45% for Staphylococcus aureus, Coagulase negative Staphylococci, Streptococcus spp., E.coli and Aspergillus fumigatus, respectively.
The results revealed that the mean values of SCC of FL quarter milk samples was 4.3×105±1.2×105, but that of FR quarter milk samples was 3.8×105 ±1.1×105,while SCC of HL quarter milk samples was 2.4×105±9.5×104 and SCC of HR quarter milk samples was 2.2×105±7.9×104.The highest frequency distribution of SCC was found in the quarter state as normal of FL was 68.96% and lies in the range of 1×104 - < 3.5 ×105,but the highest frequency distribution of SCC of FR was 68.96% and lies in the range of 1×104 - < 3.5 ×105.The highest frequency distribution of SCC of HL was 82.76and lies in the range of 1×104 - < 3.5 ×105,while the highest frequency distribution of SCC of HR was 86.20% and lies in the range of 1×104 - < 3.5 ×105.
The incidences of subclinical mastitis in the examined cows according to the each affected quarters% in relation to the all affected quarters (27) were 9 (33.3%), 9 (33.3%), 5 (18.5%) and 4 (14.8%) for FL, FR, HL and HR, respectively.
The agreement between score (+1) and (+2) CMT and bacteriological results were 100%, while the agreement between score (+1) and (+2) CMT and yeasts and molds results were 36.4% and 43.8% respectively.
The results revealed that 27(23.28%) were positive for CMT, SCC and bacteriological so there was a positive correlation between SCC and bacteriological status in quarter milk samples, while yeasts and molds were positive in 11(9.48%) quarters and negative in 105(90.52%).
The results revealed that the average of SNF% with high SCC % were 8.5, 8, 9and 8.9 for FL,FR,HL and HR, respectively, while the average of fat with high SCC % were 2.8,2.8, 3.2 and4.3 for FL, FR, HL and HR, respectively, while the average of lactose% with high SCC % were 4.7, 4.8, 5.1 and 4.9 for FL, FR, HL and HR, respectively but the average of total proteins% with high SCC % were 3.4, 2.9,3.4 and 3.4 for FL,FR,HL and HR, respectively.