الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
Fish is one of the most nutritive and highly desirable food stuffs as fish meat has good nutritional value being rich in vitamins, protein and unsaturated fatty acids. The most popular fresh water fish in Egypt are Oreochromis niloticus and Clarias lazera. The fish flesh, which is the main edible part, is generally sterile immediately after catching; however, it may become contaminated with different bacteria during subsequent handling as these bacteria can penetrate from skin and the gut to the flesh. So the present study was planned as an attempt to throw lights on the following items:•Isolation of bacteria from fish and human.•Biochemical and serological identification of bacteria isolated from fish and human.•Sensitivity test for isolated bacteria.•PCR for isolated bacteria.In this study, 1500 samples, 300 from each (skin swabs, gill, liver, spleen and kidney samples) were collected from 280 Tilapia (Oreochromis niloticus), 140 fish from markets and 140 fish from farms and 20 Catfish (Claris Lazera) were collected from markets. Also 50 human skin swabs were collected (25 samples from sellers in markets and 25 samples from sellers in farms). All these samples collected at Kafr- El sheikh governorate.E. coli was the predominant bacteria isolated from Tilapia (21.4%) followed by Staphylococcus spp. (19.6), Salmonella spp. (19%), Pseudomonas spp. (11.4%), Aeromonas spp. (10%) and Edwardsiella spp. (1.1%).E. coli was the predominant bacteria isolated from Catfish followed by Salmonella spp., Staphylococcus spp., Pseudomonas spp., Aeromonas spp. and there is no Edwardsiella spp. was isolated from Catfish.E. coli was the predominant bacteria were isolated from human (24%), (20%) of isolates were from sellers at markets and (28%) from workers at farms. Followed by Staphylococcus spp. (22%), (24%) of isolates were from sellers at markets and (20%) from workers at farms. Salmonella spp. (20%), (16%) of isolates from sellers at markets and (24%) from workers at farms. Pseudomonas spp. (12%), (16%) of isolates from sellers at markets and (8%) from workers at farms. No Aeromonas spp. and Edwardsiella spp. were isolated from human.Ten E. coli isolates were serotyped (six from Tilapia and four from human). serotyping revealed that five isolates from Tilapia were belonging to (O153, O1, O125 and 2 O78). Three isolates from human belonging to (O153, O26 and O78).Ten Salmonella isolates were serotyped (six from Tilapia fish and four from human). Serotyping revealed that four isolates from Tilapia belonging to S. Inganda, S. Typhimurium, S. Kentucky and S. Molade. Two isolates from human belonging to S. Typhimurium and S. Enteritidis.Most strains of Staphylococcus aureus were sensitive to pencillin, erythromycin, flumequine and sulphamethoxazol + trimethoprim.Most isolates of Staphylococcus aureus were resistant to ciprofloxacin and chloramphenicol.Most isolates of Staphylococcus aureus were moderately sensitive to neomycin.E. coli O153 isolates from fish were resistant to doxycyllin, flumequine and sulphamethoxazol + trimethoprim while O153 isolates from human were resistant to erythromycin and doxycyclin and these isolates were moderately sensitive to ciprofloxacin, chloramphenicol and flumequine.E. coli O1 isolates were resistant to ciprofloxacin, chloramphenicol, flumequine and sulphamethoxazol + trimethoprim.E. coliO125 isolates were resistant to ciprofloxacin, chloramphenicol and doxycyclin. These isolates were moderately sensitive to flumequine.E. coli O78 isolates from fish were resistant to erythromycin and doxycyclin.E. coli O78 isolates from human were resistant to ciprofloxacin and chloramphenicol.E. coli O26 isolates were resistant to Erythromycin and doxycyclin.S. Inganda was resistant to pencillin, erythromycin and neomycin.S. Typhimurium isolates were resistant to Pencillin, chloramphenicol and erythromycin. These isolates were moderately sensitive to amoxicillin, ciprofloxacin, neomycin and doxycyclin. These isolates were sensitive to flumequine and sulphamethoxazol + trimethoprim.S. Typhimurium isolates from human were resistant to ciprofloxacin and moderately sensitive to neomycin.