الفهرس | Only 14 pages are availabe for public view |
Abstract This study aimed to isolate a beneficial nitrogen fixing Rhizobium from root nodules of faba bean (Vicia faba L. Giza 843). After series of confirmation and biochemical tests, it was identified as Rhizobium leguminosarum bv. Viciae. The isolation of Rhizobium was made on the base of the ability to form active nitrogen-fixing nodules in an alkaline soil. Three sequenced screening laboratory experiments were carried out for selecting some plant growth promoting fungi (PGPF) on the base of cellulase activity, phosphate solubilization and indole-3-acetic acid production. Two field experiments were conducted at Tag Elezz research station, Dakahlia Governorate, in two successive seasons; 2012/2013 and 2013/2014, to study the effect of co-inoculation of isolated Rhizobium (R) with selected PGPF on nodulation, growth and yield of faba bean plants. All dual inoculations used in second season led to significant increases in seed yield per feddan than single inoculation by R leguminosarum. The best four values of seed yield per feddan were observed by Saccharomyces cerevisiae + R, Trichoderma harzianum + R, Pleurotus columbinus (spent mushroom straw) + R and Penicillium rugulosum AT34 + R with non-significant difference among them and the percentage of increase over sole inoculation by R were 25.08, 23.31, 21.43 and 20.47 (%), respectively. Therefore, these fungi are considered as new promising plant growth promoters and the commercial production of an inoculum based on dual inoculation of them with R should be encouraged, especially in alkaline soils. |