الفهرس 
AbstractOnly 14 pages are availabe for public view
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Abstract
Summary and Conclusion
Mesenchymal stem cells (MSCs) represent a promising tool for new clinical concepts in supporting cellular therapy. Bone marrow (BM) was the first source reported to contain MSCs. However, for clinical use, BM aspiration is highly invasive donation procedure and there is decline in MSCs number and differentiation potential with increasing age. More recently, cord blood (CB) and also cord matrix (CM), attainable by a less invasive method, were introduced as alternative sources for MSCs. Another promising source is adipose tissue (AT) which is abundant in large volumes and relatively easier to obtain than BM.
The present study was intended to compare cultured MSCs from (BM, AT, CB, and CM) in terms of their (1) morphology, (2) potential to proliferate and expand under standardized culture conditions by measuring known surface markers (CD34, CD133, CD90, and CD105), and (3) viability by estimation of 7AAD using flow cytometer.
The present study was carried out on 24 samples (6 from each tissue type), collected from obstetric and orthopedic departments in Dar Elsalam and Elmonira hospitals, Cairo, Egypt.
MSCs of the adipose tissue, and cord matrix were isolated by mechanical-enzymatic protocol where trypsin was the enzyme of choice whereas BM-MSCs were got by density gradient separation method by layering the BM aspirate on Ficoll solution (density: 1.077 g/ml) then centrifugation was done to separate the buffy coat layer which contains the MSCs from the whole sample. Automated cell separator (AXP AutoXpress) was used for cord blood samples. This automated separator is believed to harvest about 97% of the mono nuclear cells (MNCs) present in the samples.
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Summary and Conclusion
After isolation, MSCs were cultured at 90 ml culture flasks in static, serum added liquid culture for 6 days with medium change in the middle of this period. Culture medium was prepared from RPMI 1640 supplemented with fetal bovine serum (FBS) (ratio 5:1) with addition of Penicillin/ Streptomycin/ Amphotericin B to avoid contamination. All tissue culture flasks were incubated at 37ºC at 5% CO2 incubator.
Our results denote that MSCs’ morphology is almost the same regarding the four tissue types as they all had the fibroblast like shape, CB-MSCs is preferable than BM-MSCs regarding markers positivity, and BM-MSCs is better than CM-MSCs regarding viability.
In the latter, MSCs’ field is developing in a tremendous way and multiple new sources are intensely investigated. Taking into account all the advantages and disadvantages of the four sources discussed above, depending on the therapeutic indication, the clinical applications may be based on differentiation capacity, but more likely on the abundance, frequency, and expansion potential of the cells.