الفهرس 
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Abstract
In this study, the aim was to determine the infestation rate of intestinal nematodes in domestic dogs in Sohag and Luxor, and their impact on health and different hematological and biochemical parameters. A total number of 120 domesticated dogs were examined for intestinal nematodes from private clinic “Vet Clinic Sohag” in Sohag, and “Animal Care Hospital” at Luxor governorate, southern Egypt. During the period from May 2016 till April 2017. Dogs were of different ages from 1 month to 3 years, both sex and of different breed’s size.
Fecal sampleswere taken directly from recti from 120 dogs for macroscopic and microscopic examination, three types of intestinal nematodes (Toxocara canis, Toxascaris leonina and Ancylostoma caninum) were detected.
The clinical symptoms observed in the infested dogs were highly visible in puppies including emaciation, potbellied appearance, diarrhea, dull hair coat, hair shedding, off food, pale mucous membrane, abdominal bloating and vomiting.
An overall infestation rate of intestinal nematodes was found to be 32.5% (39/120) in this study, Three types of intestinal nematodes were detected, in particular the most prevalent nematode parasite in the study was Toxocara Canis (23.3%) followed by Toxascaris leonina (4.2%) and the least nematode was Ancylostoma caninum (3.3%), Mixed infection caused by two species (Toxocara canis and Dipylidium caninum) was recorded in (1.7%) of all dogs.
According to age, puppies (under 3 months) was the most affected age with intestinal nematodes parasites (54.8%), while infestation rate recorded (26.3%) in dogs (from 3- 6 months) and recorded (15%) in dogs above 6 months. Toxocara canis was the most intestinal nematodes infest in examined dog. It showed significant infestation rate in dogs under 3 months age 45.2%, and Toxascaris leonina was high prevalence in puppies under 3 month 7.1%, while Ancylostoma caninum was recorded in older dogs.
No significant variation between males and females in infestation rate. According to breed size there was non-significant variation between large sized breeds and small sized breeds.
The highest prevalence of intestinal nematodes in dogs was during autumn 43.8% followed by winter 42.3% while lowest prevalence in summer and spring 25% and 22% respectively.
A total of 120 blood and serum samples were collected from the cephalic vain examined for hematological and biochemical analysis. Hematological data generated from 35 blood samples of infested group (B) and of 40 samples of healthy group (A) dog populations. The levels of erythrocytic parameters (Hb, PCV and TEC) were significantly lower (P>0.05) in the infested group than in healthy group of animals, whereas group differences for TLC , neutrophils, monocytes, lymphocytes, eosinophils and basophils were non-significant. A significant increase (P >0.05) in platelets, were also observed in parasitized compared to non-parasitized animals. MCV, MCH and MCHC have non-significant variation (P <0.05).
Biochemical data generated from 35 serum samples of infested group (B) and of 40 samples of healthy group (A) dog populations was taken in a test tube (plain tube) without anticoagulant for estimation of serum biochemical parameters of affected dogs: Total protein (TSP) value for infested group was found to be significantly (P>0.01) lower than healthy group . Both Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) values were significantly increased (P>0.01) in infested group than healthy group respectively. A highly significant increase (P>0.01) in alkaline phosphatase in infested group than healthy group.