الفهرس | Only 14 pages are availabe for public view |
Abstract The present study was conduct to evaluate the effect of feeding dietary rice bran oil alone or mixed with sunflower seed oil to normal and atherogenic male albino rats on lipid profile improvement, restricting cardiovascular disease, oxidative stress, liver and heart function enhancement and histopathological changes in rats liver and heart tissues. This study was carried out using 80 adult male albino rats of Sprague-Dawley strain weighing 100-120g (116 g ±5.36) divided into 8 groups as following: group I: Rats fed on the basal standard diet (considered as negative control group -ve) group II: Rats fed on the basal standard diet supplemented with 10% rice bran oil group III: Rats fed on the basal standard diet supplemented with 5% rice bran oil and 5% sun flower oil. group IV: Rats fed on the basal standard diet supplemented with 3% rice bran oil and 7% sun flower oil. group V: Rats fed on atherogenic diet (considered as a positive control group +ve). group VI: Rats fed on atherogenic diet supplemented with 10% rice bran oil . Summary 192 group VII: Rats fed on atherogenic diet supplemented with 5% rice bran oil and 5% sun flower oil. group VIII: Rats fed on atherogenic diet supplemented with 3% rice bran oil and 7% sun flower oil Food and water were provided ad-libitum for ten weeks. The consumed foods and body weights of rats were recorded twice weekly to monitor body weight changes feed intake and feed efficiency ratio during the feeding peroid. Blood samples which collected from the portal hepaticvein were centrifuged to separat serum. Some biochemical investigations were done on serum and tissue of liver. Results of this study could be summarized as follows A) Biology study Body weight gain Body weight gain and feed efficiency ratio in rats fed on atherogenic diet showed significant elevation as compared to standard diet fed rats negative control. However, feed intake was significantly reduced in rats fed on atherogenic diet as compared to negative control. Rats body weight gain and feed efficiency ratio were significantly reduced in rats fed on atherogenic diet supplemented with 10% RBO. While, there feed intake was significantly increase as compared to positive control. Summary 193 Feed efficiency ratio in rats fed on atherogenic diet supplemented with 5% RBO exhibited significant elevation as compared to positive control. Relative organs weight Liver relative weight was significantly increased in rats fed on atherogenic diet while, heart relative weight in atherogenic diet fed rats was significantly decrease as compared by negative control. Addition of rice bran oil to atherogenic diet by different percentage (10% and 5%)significantly increase the heart relative weight while the relative liver weight was significantly decrease as compared by positive control. B) Biochemical study Serum lipid profiles The lipid profiles (TC, LDL-C, TG and VLDL-C) were significantly increased in rats feeding on atherogenic diet while addition RBO to standard diet showed significant decrease in rats lipid profiles as compared to negative control. Rice bran oil addition by 10%, 5%, 3% to the rats fed on atherogenic diet was significantly decreased serum TC, LDLC, TG and VLDL-C as compared to positive control while significantly increased in HDL-C by respectively as compared by positive control. Summary 194 Serum free fatty acid and atherogenic factors Rats fed on atherogenic diet exhibited significant elevation in serum FFA, TC/HDL-C ratio and LDL-C/HDLC ratio compared to negative control. Addition of rice bran oil to atherogenic diet by different percentage 10%, 5%, 3% significantly decrease the serum FFA, TC/HDL-C ratio and LDL-C/HDL-C ratio as compared to positive control. Lipoprotein electrophoresis: The Alpa- lipoprotein percentage % (HDL-C%) was significantly decreased while Beta-lipoprotein percentage% (LDL-C%) was significantly increased in atherogenic rats as compared to negative control. Addition of rice bran oil to atherogenic diet by different percentage 10%, 5%, 3% was significantly decreased the prebeta- lipoprotein (V-LDL-C %) percentage as compared to negative control. However, addition of RBO by 10%, 5%, 3% to atherogenic diet exhibited significant reducetion in Betalipoprotein percentage and prebeta- lipoprotein percentage while Alpa-LP % was significantly increase as compared to positive control. Serum and liver lipid peroxidation (MDA) and antioxidant capacity: Feeding on atherogenic diet raised significantly serum (MDA) and tissue (MDA) while the total antioxidant Summary 195 capacity was significantly decreased as compared to negative control. Addition of RBO to standard diet or atherogenic diet showed highly elevation in serum total antioxidant capacity as compared to negative control. Feeding on atherogenic diet supplemented with 10%, 5%, 3%RBO exhibited significant reduction in serum (MDA) and tissue (MDA) while, serum total antioxidant capacity was significantly increased as compared to positive control. Heart functions Serum LDH, CK-MB activities were significantly increased in atherogenic diet fed rats as compared to negative control. While atherogenic diet supplemented with 10%, 5%, 3% RBO exhibited significant decrease in serum LDH, CKMB as compared with positive control. Liver function: The results exhibited significant elevations in ALP, ALT and AST activities in atherogenic diet fed rats as compared to negative control group. While a significant decreased in liver functions were observed in the rats fed on atherogenic diet supplemented with different percentage of 10%, 5%, 3%RBO as compared by positive control. It was observed that serum concentrations of total protein, albumin and globulin were decreased in rats fed on atherogenic diet and atherogenic diet supplemented with different percentage of RBO as comparison to negative Summary 196 control.However, Supplementation the atherogenic diet with RBO was elevated level albumin and total protein as compared to positive control Kidney functions: Serum urea and creatinine levels were significantly increased by feeding on atherogenic diet as compared to negative control. Serum urea and creatinine showed non significantly difference in rats fed on standard diet and atherogenic diet with different dietary treatment 10 %, 5% and 3% RBO as with compared to either the negative or positive controls. C) Histological studies: Histopathological changes of liver and heart: The heart of atherogenic rats showed congestion of myocardial blood vessels and at some section showed focal necrosis of cardiac myocytes while the heart section from negative control rats and RBO fed rats revealed the normal histological structure.The liver of atherogenic rats showed small vacuoles in the cytoplasm of heptocytes and sinusoidal leucocytosis while the liver sections of the rats fed on rice bran oil showed no histopathological with activation of kuffer cells. |