الفهرس 
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Abstract
Summary And Conclusion
Blastocystis spp. is an anaerobic, cosmopolitan, eukaryotic, and enteric protozoan that inhabits human intestinal tract, it has a widespread geographic distribution in countries of all income levels globally. A true pathogenic status of B. hominis is yet controversial, while it has been found in patients with gastrointestinal symptoms mainly. Moreover, B. hominis is usually found in people who report no symptoms, and it has been estimated that most cases may be asympyomatic.
B. hominis infection is more common in developing countries in the tropical and subtropical regions. Different surveys have pointed out that the infection rate can vary from 1.6% in industrialized countries to more than 50% in developing countries, and inferior socioeconomic groups and those living along with low values of hygiene showed elevated incidence rates than the other group of the community.
The aim of this study is finding if there is statistically significant relation between B. hominis and other intestinal parasitic infections. 150 stool samples were collected from outpatient clinics of some MOH hospitals and units in Sohag City. Stool samples were examined for presence of B. hominis and other parasites using formol ether sedimentation technique with modified ZN, and Trichrome staining technique.
In this study 33 samples (22%) were positive for presence of parasites, while 117 samples (78%) were negative. Out of 33 cases, only 25 cases (75.8%) were positive for presence of B. hominis either single or mixed with other parasites and 8 cases (24.2%) were positive for parasites other than B. hominis. Out of 25 cases, only 9 cases (36%) were infected with B. hominis alone, while 16 cases (64%) were infected with B. hominis and other parasites.
Statistical analysis of the presently obtained results showed that there are significant relations between B. hominis and Giardia lamblia, E. coli. On the other hand, statistically non-significat relation was found between B. hominis and E. histolytica and H. nana.
Staining trial with acid fast stain failed, but the parasite was stained well by Trichrome stain. The trichrome stain gave the same result in identification as the iodine stained smears. from these results it can be reported that B. hominis can be easily identified in stool samples by iodine stained smear after concentration by Formol-ether sedimentation technique as it is simple, rapid and less time consuming technique than Trichrome staining technique as it more expensive and time consuming technique, so that the same results can be obtained by direct smear rather than Trichrome stain.
In Conclusion:
B. hominis and other parasites infection are more common in developing countries than industrialized countries and this can be referred to low socio-economic standard, health education, personal hygiene, overcrowding and sanitary disposal of human excreta.
There is statistical significance in association between B. hominis with G.lamblia and E. coli and this mean the infection with G. lamblia and E. coli more common with B. hominis than other parasitic infection. This may be due to symmetry in genetic characters of these parasites or may be due to these parasites need the same environmental conditions. To confirm this association and its reasons we need for further investigations with PCR- based methods in the future.