Search In this Thesis
   Search In this Thesis  
العنوان
Histological study on the effect of formaldehyde on the ovary of adult female albino rats and the possible protective effect of Omega-3 /
المؤلف
Esa, Walaa Abdellah Ismail Ibrahim.
هيئة الاعداد
باحث / ولاء عبد اللاه إسماعيل إبراهيم
مشرف / مها السيد سليمان
مشرف / أحمد أبوزيد على
مشرف / ماجده أحمد منصور
الموضوع
Omega-3 fatty acids - Therapeutic use.
تاريخ النشر
2018.
عدد الصفحات
179 p. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
علم الأنسجة
تاريخ الإجازة
29/1/2018
مكان الإجازة
جامعة المنوفية - كلية الطب - الهستولوجي
الفهرس
Only 14 pages are availabe for public view

from 179

from 179

Abstract

Human reproduction is a delicate process that can be influenced by many factors.
Reproductive toxicity broadly refers to the occurrence of biologically adverse effects on the reproductive system that may result from chemical exposure to environmental agents and is characterized by alterations in the female or male reproductive organs, related endocrine system, and pregnancy outcomes.
Formaldehyde is a flammable, colourless gas with pungent odour and readily polymerized at ambient temperature. It is one of the major pollutants in indoor and outdoor air. It is found in the environment as a result of natural processes and from man-made sources and it is widely used in industries and medical settings. Omega-3 (n-3) is an essential fatty acid (EFA,) found in large amounts in fish oil. It contains eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Omega-3 is found also in vegetable sources such as soybean and linseed oil.
Recent studies documented that Omega-3 had antioxidant, antiapoptotic and antiinflammatory effects on various tissues so it is hopeful to be used as potential cytoprotective agent against various extrinsic toxic stimuli.
This work was carried out to study the histological, immunohistochemical and morphometrical changes of formaldehyde on the ovary of adult female albino rats and to clarify the possible protective effect of omega-3 on these changes.
Materials and Methods:
Sixty adult female albino rats were used in this study. They were housed in stainless steel cages at room temperature. Rats were randomly divided into four main groups: The rats were randomly divided into four main groups: group I (control group): composed of 10 adult female rats keptwithout any treatment.group II (Omega -3 treated group): composed of 10 adult femalerats that were administered omega -3 at a dose of 400mg/kg dailyvia intragastric gavage for 14 days.group III (Formaldehyde treated group): composed of 20 adultfemale rats which were subdivided into two equal subgroups:
Subgroup IIIA (low dose formaldehyde treated rats): wascomposed of 10 adult female rats that received formaldehyde at adose of (2 mg/kg/day) for 14 days via intraperitoneal injection.Subgroup IIIB (high dose formaldehyde treated rats): wascomposed of 10 adult female rats. They received formaldehyde at a dose of (20 mg/kg/day) for 14 days via intraperitoneal injection.
 group VI (combined formaldehyde and omega-3 treated group):
Composed of 20 adult female rats which were further subdivided into two equal subgroups:
Subgroup VIA (combined low dose formaldehyde and omega-3treated rats): was composed of 10 adult female rats. Theyreceived formaldehyde at a dose of (2 mg/kg/day) viaintraperitoneal injection concomitantly with omega-3 that wasadministered at a dose of 400mg/kg daily via intragastric gavage for 14 days
Subgroup VIB (combined high dose formaldehyde and omega-3 treated rats): was composed of 10 adult female rats. They received formaldehyde at a dose of (20 mg/kg/day) via intraperitoneal injection concomitantly with omega-3 that was administered at a dose of 400mg/kg daily via intragastric gavage for 14 days
At the end of the determined period, animals were sacrificed, and both ovaries of each animal were excised. The weight of ovaries was measured then were fixed in 10% formol saline& processed for paraffin blocks. Five micrometer sections were cut and stained with (Hx. &E.) and Mallory’s trichrome (M.T) stain for routine histological examination and with beta estrogen receptor, progesterone receptors and PCNA for immunohistochemical study. Morphometric study and statistical analysis were also done.