الفهرس 
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Abstract
Pseudomonas aeruginosa is a Gram negative bacterium, oxidase positive, isolated from fish and can be found in human. Samples were taken from freshly caught 165 live fishes, which were randomly collected from fresh water farms. where they were two kinds of fishes: 1- Ninty Tilapia niloticus spp. (Orechromis niloticus). 2- Seventy five Karmout catfish spp. (Clarias geripinis). Which showed a signs of septicemia. Also, a total number of 120 human samples (60 urine, 40 sputum and 20 pus samples) were collected from patients from Suez Canal university hospital. Samples were obtained to: 1- Isolate and identify P. aeruginosa. 2- Investigate some of phenotypic characters of P. aeruginosa in a comparative study to isolated strains from different sources of origin (fishes and human). 3- In vitro antibiotic sensitivity testing was done as a way to control P. aeruginosa infection. 4- Resistant factors associated with these isolates were detected which lead to resistance to antibiotics through detection of two specific genes related to efflux pump (mexA-mexB) by using PCR. The present study revealed that: The percent of isolation of P. aeruginosa were 23.3 %, 52%, and 25% were recovered from Tilapia (Oreochromis niloticus), Catfish (Clarias gariepinus), and human samples respectively. Increasing the percent of isolates from kidney than other organs and tissue in Tilapia (Oreochromis niloticus) with percentage of 10%. While in Catfish (Clarias gariepinus) increasing the percent of isolates from liver with percentage of 28%. Pseudomonas aeruginosa was cultivated on Cetrimide agar media to detect pyocyanine pigment. Phenotypic comparative study of isolated strains from different sources of origin (fishes and human) revealed that all isolates were typical in their morphological, culture and biochemical characters. In vitro antibiotic sensitivity test to find the antibiogram pattern for isolated strains, it was found that P. aeruginosa of human origin is only sensitive to Norofloxacin (20%).While P. aeruginosa of fish origin is nearly resistant to most of used antibiotics. PCR results showed the percent of presence (mexA-mexB) genes in tested isolated strains from fish and human origin were