الفهرس 
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Abstract
A total of 400 individual quarter milk samples were collected from 100 apparently healthy cows and buffaloes, were examined to detect the incidence of subclinical mastitis using CMT, and confirmed by lactose, chloride and milk enzymes, positive samples were subjected to microbiological examination represented in counting, isolation and identification of some pathogens of public health importance.
The results can be summarized as the following:
Incidence of subclinical mastitis:
Results revealed that 25.5% of examined quarter milk samples were positive to screening test used.
Staphylococcus aureuscount:
Results were ranged from 6×102 to5×106 with a mean count of 8.7〖×10〗^2 ± 2.8×〖10〗^5 cfu / g. and incidence of Staph.aureus in examined samples were 90.20%.
3-Staphylococcus epidermidis count:
Results were ranged from 2×102 to 1×105 with mean count of 1.9×〖10〗^4 ±1.7×〖10〗^2cfu / g. and incidence of Staph. epidermidisin examined samples were17.65%.
4-Streptococcus spp. count:
Results were ranged from 1×102 to 2×109 with a mean count of 2.6×〖10〗^7±1.5×〖10〗^2cfu / g. and incidence of Strept. spp. in examined samples were 77.45%.
4.1.Strept. Pyogen: incidence was 43.14% in examined quarter milk samples.
4.2.Strept. agalactiae:incidence was 29.41% in examined quarter milk samples.
4.3. Other undefined Strept.spp. :Incidence was 29.41% in examined quarter milk samples.
5-E.coli count:Results were range from 1×102 to 1.3×106 with a mean count of 8.4×〖10〗^4±0.53 ×〖10〗^2cfu / g. and incidence of E.coliin examined samples were 34.31%.
6- Yeast and Mould count: Results were range from 1×102 to 1×107 with a mean count of 1.7×105 ±1×102cfu / g.
7- Chemical and enzymatic examination of mastitis milk samples:
7.1. Lactose%: Results were range from 2.23 to 5.7 with a mean count of 3.16 ± 0.79.
7.2. Chloride%:Results were range from 0.07 to 0.24 with a mean count of 0.13 ± 0.19.
7.3. ALP %:Results were range from 276.18 to 2561with a mean count of 723.769 ± 21.348.
7.4. LDH%:Results were range from 165 to 844 with a meancount of 503.52 ± 14.215.
Correlation coefficient were applied and found that there were significant correlation between chloride and ALP,LDH enzymes while non significant correlation between lactose and ALP,LDH enzymes.
The public health importance of isolated organism as well as the control measures recommended to control subclinical mastitis were discussed.