الفهرس 
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Abstract
Rabies is the most serious and most feared infectious disease affecting human and animals. The currently available types of anti-rabies antibodies have many disadvantages. The present work provides an attempt to produce anti-rabies immunoglobulin in chicken egg yolk. In this study, a total number of 40 egg laying hens were involved where 30 birds were immunized with rabies vaccine and the remaining 10 birds were kept as a control group. Birds have received five doses of the vaccine; the first booster dose was given at one week post 1ry immunization then birds were repeatedly boosted three times at two weeks intervals. Eggs of immunized hens were collected and used for extraction of anti-rabies IgY. Three method of IgY extraction were used; chloroform, poly ethylene glycol (PEG) and chloroform-PEG methods. The three methods were compared in terms of purity and protein content, where chloroform method was found to give the highest protein yield followed by chloroform-PEG and then the PEG method. In SDS-PAGE analysis, PEG and chloroform-PEG methods were found to give better purity than chloroform method. By western blotting, the anti-chicken antibodies specifically detected the heavy and light chains of the IgY only confirming that the majority of the extracted proteins are IgY. By using ELISA, it was confirmed that the prepared IgY antibodies are specific to rabies virus, the anti-rabies antibodies were detected at two weeks post immunization and increased gradually by boostering. In vivo testing of the prepared antibodies revealed that the antibodies could neutralize the rabies virus infectivity in mice experimentally infected with challenge virus standard (CVS). At 9th week post immunization the antibodies could neutralized 100 LD50 of CVS and protected 100% of inoculated mice. ELISA could be used for calculating antibodies titer instead of the currently used neutralization technique after calculating a correction factor between ELISA and neutralization.