الفهرس 
covers englishOnly 14 pages are availabe for public view
 |  | from | 142 |  |  |
| | | from | 142 | | |
Abstract
The genetic markers associated with inflammatory responses during mastitis afford a reasonable route for improving milk production in Egyptian buffalo breed. Among these markers are the interleukin 8 receptor B gene; a chemokine receptor gene required for targeting neutrophil migration during infection. To better understand its role during mastitis in Egyptian buffaloes, twenty five dairy animals representing the normal, sub-clinically, clinically and chronically affected buffaloes were randomly selected from different districts. Buffaloes were screened for mastitis based on somatic cell count and California mastitis test assays using their milk samples. Two overlapping primer pairs, amplifying interleukin 8 Receptor B gene exon2, were designed. Sequences of polymerase chain reactions products were investigated and the deduced sequence was 1246bp and was submitted to Genbank (accession # KY399457.1). It covers part of intron1, exon2 and a part of 3’UTR. The study clearly defined the presence of four SNPs. Three were detected as synonymous substitutions in coding region C/A c.127, C/T c.546 and C/A c.562 and one A/G c.1092+g.62 in the 3’UTR region. Only SNP C/A at c.127 was found to be highly associated with mastitis. Only the c.546 SNP, at the codon 182, was located in the 4th transmembrane receptor. Biochemically, the mastitic animals showed increase in milk enzymes like lactate dehydrogenase, alkaline phosphatase and serum malondialdehyde. In addition, the total antioxidant concentrations, however, decreased in serum and milk in response to mammary inflammation. The protein profiling of milk whey showed an increased protein concentration. In conclusion, the results warrant the potential correlation between the genetic SNP variance for certain genes and the incidence of mastitis in buffalo breed.