الفهرس 
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Abstract
Background and objectives: Liver transplantation is one of the greatest advances of the past 3 decades for the care of patients with hepatic failure. Innate immune defenses against infections are altered after liver transplantation. TLR-2, one of the innate immune elements, is considered as the main receptor for gram positive bacterial cell wall components. The aim of this study is to investigate post living donor liver transplantation associated infection and its relation with both serum Toll like receptors and Toll like receptor genetic polymorphism. Methods: Out of 125 subjects enrolled in our work, 109 subjects completed this study. Subjects were classified into two groups: 66 patients who undergone LDLT and 43 healthy donors as a control group. Serum TLR2 protein was measured by ELISA technique in the serum of the recipients pre and post transplantation and also measured in the serum of the donors before transplantation. Also, SNPs analysis of TLR2 gene during pre-operative assessment was done at 3 SNPs for both recipients and donors. The association between TLR2 SNP and serum TLR2 protein with development of infection post LDLT was studied. Results: Serum TLR2 was significantly higher in recipients post transplant period compared with recipients and donor in pre transplant period. Conversely, no significant difference was detected in post transplant serum TLR2 of recipients on comparing non infected group with infected group regarding blood, urine and sputum cultures. By using ROC curve in order to study the validity value of serum TLR-2 in infection post LT, TLR2 had area under the curve (AUC) (0.49) with cut-off point value equals to or above 3.145 ng/ml based on comparison between infected versus non infected patients post LT. Regarding rs +597 T>C, rs5743708 +753 G>A and rs121917864 +677 C>T; T,G, C alleles are the protective alleles respectively and C, A, T alleles are the risky alleles respectively for development of infections post LT. Conclusion This study shows that the risk of developing infection post liver transplantation can be partly explained by genetic polymorphisms in TLR2 gene. Serum TLR 2 proteins cannot be used as a marker for infections post liver transplantation