الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
NCD has a devastating transboundary economic impact on cattle industry. One of possible viral causes of NCD is BNoV (genus Norovirus, family Caliciviridae). Noroviruses of bovine origin are usually present in genogroup III (GIII), which has two main genotypes; GIII.1 (Bo/Jena/1980/DE) and GIII.2 (Bo/Newbury-2/1976/UK) originally discovered in Germany and England, respectively. Further genotypes were identified later due to genetic recombinations occurred with the virus genome.
The single stranded positive sense RNA consists of 3 overlapping regions (namely; ORF1, ORF2 and ORF3). The ORF1 encodes for a polyprotein, which is cleaved post-translationally into 6 non-structural proteins, including the RdRp enzyme. The ORF2 encodes for the major structural protein (VP1) while the ORF3 encodes for the small capsid protein (VP2). The VP1 is responsible for receptor-binding and immune reactivity by its P2 subdomain. The VP2 is involved in virion stability and capsid assembly. BNoV-VP2 probably follows the VP1 in the genetic (phylogenetic) clustering and may play a role in the evolution of BNoVs.
The study was conducted to molecularly detect and phylogenetically characterize the circulating BNoV strains in Sharkia province of Egypt between late 2016 and early 2017 using RT-PCR. After extraction of viral RNA, one reference primer pair was used to target a conserved region in the RdRp.