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العنوان
Phenotypic and Molecular Studies on
Vancomycin Resistant
Staphylococcus
Species Isolated from Paediatric Hospital /
المؤلف
Hussein, Waleed Elsayed Khedr .
هيئة الاعداد
باحث / وليد السيد خضر حسين عباده
مشرف / مجدي زكي مطر
مناقش / ميساء السيد زكي
مناقش / أماني محمد أبو العينين
الموضوع
Staphylcoccus species. Infection control issues.
تاريخ النشر
2017
عدد الصفحات
124 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
علم الأحياء الدقيقة
تاريخ الإجازة
22/10/2018
مكان الإجازة
جامعة المنوفية - كلية العلوم - قسم ميكروبيولوجي
الفهرس
Only 14 pages are availabe for public view

from 140

from 140

Abstract

Some Staphylococcus species can cause a wide variety of diseases in humans and animals. Multi-drug resistant organisms including Staphylococcusspecies persist and spread worldwide Vancomycin has long been the preferred treatment of multi-drug resistant
Staphylococci
. <>Unfortunately vancomycin resistance has been reported in clinical isolates of Staphylococci Staphylococcus clinical isolates were collected, cultured, isolated and identified by several biochemical
tests manually
and automatically.
Susceptibility tests for different antibiotics were
performed by different methods. DNA of
Staphylococcus
isolates was
extracted, and the presence of
vanA
gene was detected using PCR. The
vanA
gene sequence was determined. A total of 50
Staphylococcus
isolates were isolated. They contain
13
Staphylococcus species
. All
isolates produced catalase, 18% produced coagulase. Many isolates were
multi-drug resistant. 48 % of isolates had
vanA
gene. In conclusion the
rules of infec
tion control must be followed to avoid the spread of multi-
drug resistant microbes. At the same time research has to focus on
development of new antibiotics to treat the infected patients. Much work
must be done to determine the true risk factors for infec
tion and
understanding the mechanisms of vancomycin resistance