الفهرس 
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Abstract
Fabaceae are a large and economically crops that play an important role in human nutrition since they are rich sources of protein, calories, certain minerals and vitamins. In view of vital role of some endophytes which have positively influence plants vitality by promote plant growth and/or protect their host against phytopathogens.
The aim of this study was divided into three parts: * The first part was conducted in vitro and aimed to screen and identify some endophytic bacteria isolated from the roots, nodules, stems and leaves of healthy legumes and none-legumes crops.* The second part aimed to evaluate the ability of the more potent isolates to inhibit soil borne pathogenic fungi for faba bean and pea plants in greenhouse conditions.
* The third part was conducted in field to study the effect of endophytic bacterial strains in combination with either humic acid or microelements as foliar application on microbial activities in soil and its impact on growth performance and productivity of faba bean and pea plants. Obtained results can be summarized as follows:
The first part
5.1. Isolation of endophytes
A total of 167 endophytic isolates were isolated from healthy legumes and non-legume plants such as fababean (vicia faba), pea (Pisum sativum), fenugreek (Trigonella foenum-graecum), lupine (Lupinus), phaseolus (Phaseolus vulgaris) and rice (Oryza sativa).
5.2. Screening of the endophytic isolates
5.2.1. Primary screening of endophytes
The primary screening was depending on cellulase and pectinase activity
5.2.1.1. Cellulase activity
Results indicated that from 167 isolates, 118 isolates showed zone of clearance around their colonies, the clearing zone diameter ranged from 0.20- 3.50 cm. Also, it should be noticed that, 40 isolates has clear zone between 2.0-3.0 cm, 44 isolates recorded clear zone between 1.0-1.9 cm and 28 isolates has clear zone less than 1.0 cm, while, only 49 isolates did not show any noticeable clearing zone. In addition, 6 endophytic isolates number End15, End 55, End 64, End 68, End 111 and End 114, showed maximum clearance zones around their colonies over 3.0 cm. While, from 167 isolates, 146 isolates showed cellulase activity in liquid medium. Cellulase activity ranged from 0.02- 0.982 (U/ml), while about 21 isolates did not display cellulase activity.
5.2.1.2. Pectinase activity
Regarding the pectinase activity, obtained results showed that lowest clear zone ranged from1.00- 1.90 cm was obtained by 34 isolates. Total of 28 endophytic isolates out of 167 endophytic isolates were able to do a clear zone with 2.00-3.00 cm while, three isolates number End 2, End 96 and End 142 gave the highest records of clear zone over 3.00 cm. On the other hand, 102 isolates did not show any noticeable clearing zone. Whereas, from 167 endophytic isolates, 101 endophytic isolates produced pectinase in liquid medium. Also, data revealed that 66 endophytic isolates from 167 did not display pectinase activity.
5.2.2. Secondary screening of endophytic isolates 5.2.2.1. Root colonization Results emphasized that the selected endophytic isolates increased the rate of root length, shoot length, root fresh weight and log CFU inside root more than the control.