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العنوان
Evaluation of Anti-biofilm efficacy of silver nanoparticles against clinically isolated Methicillin Resistant Staphylococci /
المؤلف
Abo Shahba, Dina Shafeek Shokry.
هيئة الاعداد
باحث / دينا شفيق شكرى أبو شهبه
مشرف / محمد زكريا حصين
مشرف / هدى الصيد احمد عس
مشرف / مروة مصطفى شلبى
الموضوع
Microbiology. Medical Microbiology& Immunology.
تاريخ النشر
2017.
عدد الصفحات
p 166. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
علم الأحياء الدقيقة (الطبية)
تاريخ الإجازة
18/6/2017
مكان الإجازة
جامعة طنطا - كلية الطب - الميكروبيولوجيا والمناعة
الفهرس
Only 14 pages are availabe for public view

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Abstract

S.aureus and S.epidermedis are a diverse group of bacteria that cause diseases ranging from minor skin infections to life threatening bacteremia. MRSA and MRSE are the most multi resistant pathogens that cause infections and lead to morbidity and mortality, they have non-specific mechanisms of resistance, of which biofilm formation is the most important. The difficulty in eradicating a chronic infection associated with biofilm formation lies in the fact that biofilm bacteria are able to resist higher antibiotic concentration than bacteria in suspension.
The goal of the present work was to isolate MRSA and MRSE from different clinical samples, to detect biofilm producing strains by different methods. Also, to evaluate the effect of silver nanoparticles against biofilm producing strains of MRSA and MRSE.
This study was conducted during the period from July 2016 to February 2017 from different departments of Tanta university Hospitals. It included 122 specimens, 108 were patient samples (60 males and 48 females), their ages ranged from 5 years to 83years, and 14 specimens were from medical devices. Samples were collected from different Specimens. Isolation and identification of organisms were done according to the standard microbiological methods.
Staphylococci were 44.3% from total specimens (21.3% were S.aureus and 23% were coagulase negative staphylococci). Antimicrobial susceptibility was determined by Kirby-Bauerdisc.