الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 140 |  |  |
| | | from | 140 | | |
Abstract
Diabetes comprises a group of metabolic diseases that are characterized by hyperglycemia, which is associated with damage to and/or malfunction or failure of various organs, including the eyes, kidneys, nerves, heart, and blood vessels among others. Diabetic complications are responsible for much of its associated morbidity and mortality. The risk for development of diabetic complications increases with poor glycemic control and disease duration, although other factors such as inflammation are also likely to be important.
Monocytes show considerable heterogeneity in both phenotype and function. The classification of human peripheral blood monocytes is centered on the expression of CD14 (LPS co-receptor), and CD16 (Fcϒ receptor III). Three major subsets exist:CD14hi/++CD16-(classical),CD14hi/++CD16+(intermediate)and CD14dim/+CD16hi/++ (nonclasscial).
Classical CD14++CD16- monocytes traffic to the site of inflammation during the early stages of inflammation where they exhibit functions related to a typical inflammatory response. By contrast, nonclassical CD14+ CD16++ monocytes predominantly patrol blood vessel walls and are believed to contribute to resident macrophage populations.
The aim of this study is to evaluate the percentage of expression of CD16+monocytes and its correlation with diabetes mellitus (DM) and its complications.
This work performed on 40 patients with diabetes mellitus and 20 apparently healthy subjects working as control.
Patients were further classified according to presence or absences of diabetic complications into two groups: group (1): uncomplicated diabetic group which was composed of 20 patients, group (2): complicated diabetic group which was composed of 20 patients with matched age &sex were selected as a control group.
Candidates of the present study were subjected to clinical evaluation and determination of fasting blood sugar, serum creatinine, HbA1c, liver function test (SGOT&SGPT), S.creatinine, albumin creatinine ratio (ACR) and the percentage of expression of proinflammatory marker CD16+ within CD45+,CD14+monocytes by flow cytometry.
The results of the present work showed that:-
• Fasting blood sugar, HbA1c and ACR were significantly higher in patients with diabetes mellitus compared to control group.
• Creatinine and ACR were significantly higher in patients with DM with complications compared to patients with DM without complications.
• A significant increase of creatinine concentration and ACR were associated with risk of DM complication development in univariate analysis. While in multivariate analysis, only ACR was associated with risk of development of diabetic complications within DM patients.
• No significant correlation was detected between CD16+ expression in diabetic patients group compared to control group.
• No significant difference was found in CD16+ expression diabetic patients group with complication compared to diabetic patients group without complication.
• Higher FBG, creatinine and ACR were significantly associated with low CD16+ expression in DM with complications subgroup.
• ACR has significantly positive correlation with CD16+ expression in DM without complication subgroup.
Conclusion:
The current study found that the percentage of expression of CD16+ monocytes was insignificant marker for discrimination between diabetic patients with complication & diabetic patients without complication.
So the percentage of expression of CD16+ monocytes in a diabetic patients is not a marker for prediction of diabetic complications.