الفهرس 
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Abstract
Background: Frustration affect couples when they lose their pregnancy several times successively. There are many factors that leads to Recurrent pregnancy loss, One of these factors is maternal obesity. In obese women leptin and IL-6 continuously turn on the activation of intracellular JAK-STAT3 signaling. This might lead to mutation in the gene encoding the Janus kinase2 (JAK2) that results in a replacement valine with phenylalanine at particular position 617(V617F). Which is main cause responsible for augmented rate of fetal loss in this situation.
Objectives: To explore the association of obesity with JAK2 V617F mutation in women with recurrent pregnancy loss.
Patients and Methods: This case control study included 250 female patients. The study group consists of 150 female patients with history of recurrent pregnancy loss for two consecutive times or more, aged (20-34) and having a body mass index of >24.9. The control group consists of 100 aged matched women have normal body mass index and as well as having a minimum of one live child birth and no past history of prior pregnancy loss or uncomplicated pregnancy. All women were investigated for the mutations by using the allele-specific multiplex PCR technique to genotype all participants in the study.
Results: Jak2v617 mutation was found in cases (9.3%) while no jak2 v617f mutation was found among the control group,(p< 0.001). Patients in case group were obese with a mean BMI of (30.9 ±4.3) vs mean BMI control group (22.4 ±1.1, p<0.001).History of live birth in case group (42.7%) vs 100 %in control,(P< 0.001). consanguineous marriages were positive (57.4% vs 13 %, p<0.001). Only 13 patients of the case group experienced pregnancy loss after 12 weeks of gestation while the rest of the case group (137) including 14 patients who were positive for jak2 v617f mutation experienced pregnancy loss before 12 weeks of gestation (p <0.06).
Conclusion: In conclusion we found a correlation between the increased risk for the occurrence of RPL in obese women and V617F mutation in the Jak2 gene exon 12 and that obesity could be a risk modifier for somatic mutation.
Keywords: Recurrent pregnancy loss •mutation • jak2. Obesity.