الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 152 |  |  |
| | | from | 152 | | |
Abstract
Poultry are one of the major source of animal protein in most countries worldwide, Salmonellosis is a serious problem that affects poultry industry causing economic losses and infection in man.
So the aim of the current study was planned to fulfill the following:
1-Isolation of Salmonella spp. from chickens, chicken products and humans.
2-Phenotyping of the isolates including:
- Morphological characterization (microscopic and colonial characters)
-Biochemical identification
- Serotyping 3-Antibiotic sensitivity test for the isolates 4-Widal test for diagnosis of enteric fever
5- Detection of some virulent genes in Salmonella isolates
A total of 245 samples that included 36 broilers cloacal swabs, 17 intestinal swabs, 45 chicken meat{ breast, thigh, wing, 15 of each (sample and swab ) }, 60 chicken products { luncheon, nuggets , kofta, pane, 15 of each ( sample and swab ) } were collected from chicken farms, shops and supermarkets.
Moreovere 20 hand swabs , 27 stool (diarrheic and non- diarrheic ) and 40 serum were collected from humans either contact or non-contact from hospital and private labs in Qualyobia province.
Bacteriological and biochemical identification revealed that Salmonella species were detected in 16.66 % , 15.55% , 3.33% 17.6%, 20%, 14.8% and 12.5% in examined cloacal swabs, chicken meat, chicken products, intestinal swabs, hand swabs, stool and serum, respectively.Serotyping revealed that (S. Gallinarum, S. Typhimurium, S. Tsevie and S. subspp. salamae) were isolated from broilers cloacal swabs, (S. Papauna, S. Colindale, S. Enteritidis, S. Kentucky) were isolated from chicken meat, (S. Enteritidis) was isolated from chicken products, (S. Kentucky, S. Papauna , S. Enteritidis ) were isolated from intestinal swabs, (S. Papauna, S. Enteritidis, S. Lagos, S. Tsevie) were isolated from hand swabs, (S. Kentucky, S. Typhimurium , S. Enteritidis) were isolated from stool.
Widal test revealed that S. Typhi and Paratyphi A and B were isolated from serum by a highest titer of 1:320.
Out of the isolated Salmonella strains, nine strains were tested for the antibiotics sensitivity against 6 antibiotics (ampicillin, tertracycline , cefexime , levofloxacin , amikacin, erythromycin ) by disc diffusion method, isolates were classified are susceptible, intermediate and resistant, all isolates (9 strains) were sensitive to levofloxacin and amikacin (100%) while were resistant to erythromycin (100%) and 6 strains were sensitive to ampicillin (66.6%) while one strain was intermediate (11%) and 2 strains were resistant (22%), 5 strains were sensitive to tetracycline (55.5%) while 4 strains were intermediate (44%) and 3 strains were sensitive to cefexime (33%) while 5 strains were intermediate (55.5%) and one strain was resistant (11%).from the study there is a relationship between isolated strains from humans and chickens that indicated cross contamination and chickens is a main source of Salmonella in humans.