الفهرس 
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Abstract
The aim of this study is to develop and synthesize some new compounds containing thiazolo[4,3-b][1,3,4]thiadiazole moieties in order to further assess the pharmacological activity of this class of compounds. The present work focuses on with the synthesis of these compounds as well as to evaluate their antimicrobial activity, via exploiting the reaction of 5-phenyl-5H-thiazolo[4,3-b][1,3,4]thiadiazol-2-amine (1) and its derivatives towards various reagents. Therefore, the sequence of these reactions followed for the preparation of the designed compounds in this thesis are summarized in scheme 1-9. Also includes geometry optimization of the molecular structures for different compounds was done using the DFT (density functional theory) method via cluster calculations using DMOL3 program in material studio package in order to investigate their stability relative to each other. In vitro antimicrobial screening of compounds 1- 20 prepared in this study was carried out using cultures of two bacteria species, namely, Gram positive bacteria, Bacillus subtilis (RCMB 000107, BS), Gram negative bacteria, Escherichia coli (RCMB 000103, EC) as well as one fungal strain, Candida albicans (RCMB 005002, CA). Clotrimazole as an antifungal agent and antibiotic ampicillin as an antibacterial agent for Gram positive bacteria and Gram negative bacteria were used as references evaluate the potency of the tested compounds under the same conditions. Compounds 2, 3, 6, 8, 9, 11, 12, 13, 17 and 18 exhibited the highest inhibitory activity against Gram-positive bacteria B. Subtilis. Eight analogs namely compounds 2-6, 8, 9 and 11 demonstrated the highest inhibitory activity against the fungal species C.