الفهرس 
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Abstract
The present study was carried out in plant tissue Culture Laboratory of Vegetable and Floriculture Department, Faculty of Agriculture, Mansoura University, Egypt throughout the period from 2014 to 2016. To study the effect of 6- benzylaminopurine (BAP) at 0.5, 1.0, 2.0 and 5.0 mg/L, Naphthalene acetic acid (NAA) at 0.1 and 0.5 mg/L and their combinations added to the initiation media, and 6- benzylaminopurine (BAP) at 0.2, 1.0, 2.0 and 4.0 mg/L, Naphthalene acetic acid (NAA) at 0.1 and 0.2 mg/L and their combination added to the multiplication media, as well as different auxins (i.e., Indole-3-butyric acid (IBA), Naphthalene acetic acid (NAA) and Indole-3 acetic acid (IAA) at 0.1, 0.3, 0.5 and 1.0 mg/L applied separately in rooting media on the in vitro micropropagation and acclimatization of Dendranthema grandiflorum plants. The chrysanthemum cultivar used in this research was namely flyer. To evaluate the adaptation degree of chrysanthemum cultivar to culture medium composition, the single nodal explant (0.5 – 1.0 cm) were surface sterilized and culture on the MS medium supplemented with sucrose (30 g/L), agar (7 g/L). The initiation shoots were excised and cultured on multiplication and rooting media. The obtained results indicated that explants grown on medium containing 1.0 mg/L BAP + 0.5 mg/L NAA produced the highest shoots number . While, the explants grown on medium containing 2.0 mg/L BAP + 0.1 mg/L NAA produced the tallest shoots and internodes. Moreover, the explants grown on medium containing 1.0 mg/L BAP + 0.1 mg/L NAA produced the highest leaves number. The obtained results also indicated that, the explants grown on medium shoots medium containing 1.0 mg/L BAP+ 0.2 mg/L NAA produced highest shoots number. While, those grown on multiplication medium containing 1.0 mg/l BAP + 0.1 mg/l NAA produced the longest shoots and internodes. Finally, the shoot tips grown on medium containing 2.0 mg/l BAP + 0.1 mg/L NAA formed the highest leaves number. Moreover, rooting of multiplied shoots were achieved on the same medium used in multiplication stage except for the macro elements of MS medium used at half strength, sucrose at 20 g/land0.5 mg/l IBA which formed the roots initiation within a few days,, followed by , the medium containing1.0 mg/L IBA produced the highest roots number. While, the medium containing 0.1 mg/L IBA produced the tallest roots tallest per explants. For the acclimatization process, the combination of peat moss + perlite +vermiculite (1:1:1) showed its superiority over all the other growing media used, as it gave 100% survival percentage (%), increase in leaves number per shoots, shoots length (cm), and internodes length.