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Abstract In Egypt more than 2500 mothers die every year from problems related to pregnancy and childbirth, leaving behind 200 motherless newborns. These deaths, can be avoided if mothers have proper information about access to pregnancy care. Aim: The aim of the present study was to evaluate informational guideline about antenatal care for pregnant women in Damietta city. Design: A quasi-experimental design was utilized to conduct this study. Sample & Setting: The sample of this study consisted of 93 women from the total number 500 pregnant women attending all Maternal and Child Health Centers (MCH) at Damietta city according to inclusion criteria. Tools: two tools of data collection were used in this study: The 1 st tool: Interview Questionnaire to assess knowledge and practices of pregnant women about antenatal care, the 2nd tool was opinionnaire of pregnant women about booklet. Results: The main results of this study showed the total mean scores of knowledge of the studied sample in pre-test was (25.61±6.43), After implementation of the informational guideline .it raised to be (51.26±3.70). While The total mean score of practice in pre-test was (16.28±5 .26), raised in post-test to be (29.65±2.43). Moreover, a highly statistical significant differences were detected between pre and post test as regard knowledge and practice where p ::; 0.001 Conclusion: The study concluded that implementation of informational guideline about antenatal care improved pregnant women knowledge and practices about antenatal care. Recommendation: Based on the present findings, It was recommended the following. Provision of posters & booklets about antenatal care within the Maternal and Child Health Centers (MCH) available to see by pregnant women. Further research about different methods for maternal care education |