الفهرس 
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Abstract
Both plants L. corniculatus and L. halophilus are two species belonging to genus Lotus
that growing in Egypt. Literature survey revealed that genus Lotus is prescribed in folk
medicine as contraceptives, prophylaxis and treatment of sexually transmitted diseases and
peptic ulcers. Most of the phytochemical and biological investigations described in the
literature referred to L. corniculatus which is growing in different European countries. These
findings prompted us to pursue the phytochemical investigation of the aerial parts of L.
corniculatus, growing widly in Egypt. This study includes the isolation and identification of
different compounds of the chosen plant, using different physical, chemical and spectral
methods. Furthermore, the biological activities of L. corniculatus and L. halophilus were
investigated.
This thesis consists of three parts:
Part I: Phytochemical investigation of Lotus corniculatus
Part II: Biological activities
Part III: Comparative chromatographic screening
Part I
Phytochemical investigation of Lotus corniculatus
Fresh aerial parts of Lotus corniculatus were exhaustively extracted with 70% ethanol by
percolation. The alcoholic extract was filtered, concentrated and fractionated by successive
extraction with light petrol, chloroform, ethyl acetate and n-butanol.
Ten compounds were isolated from the different fractions of Lotus corniculatus. One
isoflavone derivative L1, one flavonoidal aglycone L2, three flavonoidal glycosides L3, L4, L5
were isolated from the ethyl acetate fraction. One flavonoidal glycoside L8, two sugars L6, L9
and two oleanane saponin glycosides L7, L10 were isolated from the n-butanol fraction.
Structure elucidation of the isolated compounds was carried out using different spectroscopic
techniques.