الفهرس 
Aim of the workOnly 14 pages are availabe for public view
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Abstract
A new prodigiosin-producing bacterium (strain AM8887) was isolated from the deep water of the Red Sea, Egypt. Classical cultural, morphological, biochemical tests and modern 16S rRNA gene sequence analysis identified this bacterium as a member of the genus Serratia. Specifically, the closest strain to the local isolate is the Serratia plymuthica AJ233433T; with a percentage similarity of 98.89%. A sequential combination of Plackett–Burman design and Central Composite Design optimized the bioprocess variables for prodigiosin production by this isolate. The optimum amounts of prodigiosin was produced when the isolate grown at 25°C on a rotary shaker (180 rpm) in a medium containing 6 g/l glycerol, 6 g/l sucrose, 1 g/l yeast extract, 15 g/l NaCl and 12 g/l fertilizer factory waste water at pH 6.5. The scaling up process from the shake flask to bioreactor level was achieved and Batch cultivation was carried out at 25°C, 180 rpm, controlled dissolved oxygen at 10 % and pH uncontrolled. The result showed a remarkable increase in PG production (7316 mg/l compared to 138.9 mg/l prior to optimization) representing approximately 70 fold increase in the amount of the product.