الفهرس 
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Abstract
The work presented in this thesis represents a comparison of the
phytochemical profiles of two plants Trigonella stellata and Trifolium
resupinatum belonging to family Fabaceae and also screening the alcoholic
extracts and different fractions of both plants for their anticancer activity.
Trigonella stellata Forssk. whole plant alcoholic extract, showing the higher
anticancer activity, is used for phytochemical investigation aimmg at
isolation and identification of different components of the biologically active
fractions using different physical, chemical and spectral methods. Also
quantitative determination of the isolated three new isoflavans by a validated
UPLC method. In addition, antidiabetic, antihyperipidemic, anti-
inflammatory, antinociceptive, antibacterial and antifungal activity testing of
the extract, fractions and some of the isolated compounds of T. stellata.
This thesis consists of four parts:
PART I
Phytochemical and anticancer screening of two members of Family
Fabaceae
A- Chromatographic screening of Trigonella stellata and Trifolium
resupinatum extracts from family Fabaceae using HPTLC technique
This study aims at comparing the chemical profile of the alcoholic extracts and different
fractions of Trigonella stellata and Trifolium resupinatum family Fabaceae. The extract and
fractions of both plants were examined using HPTLC in 3 different solvent system. Each TLC
plate was visualized under UV lamp and after spraying with vanillin sulphuric acid reagent.
Preliminary phytochemical screening of T. stellata and T. resupinatum shows that both plants are
promising candidates for further phytochemical investigation, where T. stellata is more favoured
when aiming at isolation of the more polar compounds from the ethyl acetate and butanol
fractions. These results encourage biological screening of T. stellata and T. resupinatum extract
and fractions aiming at performing a biologically guided phytochemical investigation.
B- In- Vitro anticancer screening of Trigonella stellata and Trifolium
resupinatum extracts from family Fabaceae using three cancer cell
lines
Cancer is one of the most serious diseases in humans and nowadays there is a
considerable scientific discovery of new anticancer agents from natural products. The present
study compares the cytotoxic activity of the extracts of Trigonella stellata and Trifolium
resupinatum as well as the different fractions of the alcohol extracts aiming at determining the
most active extract to be used as a platform for phytochemical -based drug discovery. The two
tested alcoholic extracts exhibited different potency of cytotoxic activity using the three different
cancer cell lines. The tested extracts exhibited the lowest ICso values using the HepG-2 compared.