الفهرس 
Colorectal Cancer (CRC)Only 14 pages are availabe for public view
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Abstract
Background and aim: Colorectal cancer(CRC) is a major cause of
morbidity and mortality throughout the world. It accounts for over 9% of
all cancer incidence. It is the third most common cancer worldwide and
the fourth most common cause of death .
We aim to Study the expression of microRNA -375 in serum and
rs4939827 SMAD-7 polymorphisms in DNA extracted from blood in
colorectal cancer patients and correlate with control subjects.
Subject and methods:Blood samples were taken from 86 patients with
colorectal cancer (different grades) and 36 normal persons to serve as a
control group.
The following were done: history taking ,general examination, CBC, liver
function test , kidney function tests and CEA.
Detection of Single nucleotide polymorphism (SNP) of SMAD7
(rs4939827) in DNA extracted from blood by Real time PCR.
microRNA -375 quantitation in serum by real time PCR.
Results:We found that the wild type (CC) genotype was higher in
controls 36.1% than in cases 15.1%(p=0.01). On the other hand there is
no statistical significance difference as regards to mutant and
heterozygotes genotypes.there is higher percentage of TT genotype in
patients than in controls (33.7% vs 22.2%, p=0.3).Although there is
higher percentage of CT genotype in patients than in controls (51.1% vs
41.7%, p=0.4). There is statistically significance difference between study
groups (case and controls) as regards to distribution of different gene
alleles with higher percentage of T-alleles in cases than controls(59.3%
vs 43.1%, p=0.02) and C-alleles in controls than patients(56.9% vs
40.6%, p=0.02).
there is statistically significance difference with p-value <0.001
between study groups as regards to gene expression of mi-RNA 375
with low mean among cases .
Conclusion:miRNA-375 is down regulated in CRC patients and can
be used as serum biomarker of CRC .Also SNP rs4939827 of