الفهرس 
Part I : IntroductionOnly 14 pages are availabe for public view
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Abstract
The presented thesis falls in four parts:
Part I: Introduction
This part provides a general introduction about the investigated compounds such as, chemical structure, pharmacological aspect and analytical review for the analytical techniques used for determination of the studied drugs in pure forms, dosage forms and biological fluids. At the end of this part, we described the vision and the aim of the suggested work.
Part II: Micellar Enhanced Spectrofluorimetric Determination of some fluoroquinolone antibacterials Through Complexation with Aluminium (III): Application to Dosage Forms and Biological Fluids
This part concerned with the development of simple, sensitive, rapid and specific spectrofluorimetric procedure for determination of Lomefloxacin, Ofloxacin, Pefloxacin, Levofloxacin and Moxifloxacin antibacterial quinolones in pharmaceuticals and biological fluids. The investigated drugs were found to react with Al3+ to produce highly fluorescent stable complexes. The fluorescence intensity could be enhanced by the addition of sodium dodecyl sulfate (SDS).
While excited at 329-365 nm, the enhanced fluorescence intensity at 416-480 nm of the system (ΔF) showed a good linear relationship within the concentration range of 14-130, 8-140, 16-130, 10-100 and 10-200 ng/ml for Lomefloxacin, Ofloxacin, Pefloxacin, Levofloxacin and Moxifloxacin respectively. The detection limit of the proposed method was 2.79 – 4.50 ng/ml for the investigated fluoroquinolones.
Different factors affecting the relative fluorescence intensity of the resulting chelates were studied and optimized.
The developed method was applied successfully for the determination of the studied drugs in their pharmaceutical dosage forms with good precision and accuracy compared to reported methods. Furthermore, the method was applied to the in-vitro and in vivo determination of the investigated fluoroquinolones in human plasma.
Part III: Development and validation of fluorescence quenching method for the sensitive determination of fluoroquinolone using eosin Y dye
This part is devoted to the development of new spectrofluorimetric method for
determination of )Ciprofloxacin, Lomefloxacin, Ofloxacin, Pefloxacin, Levofloxacin and Moxifloxacin( drugs in bulk and commercial dosage forms. The method based on the formation of a binary complex between the studied drugs and a xanthene dye, eosin Y. The quenching effect of the drugs on the native fluorescence of eosin Y was measured at 543.9 nm after excitation at 519.9 nm. The factors affecting the formation of binary complexes were studied and optimized. Calibration graphs were linear over the ranges of 60–1200, 100–1200, 50–1200, 140–1400, 90–1200 and 100–1300 ng/ml for Ciprofloxacin, Lomefloxacin, Ofloxacin, Pefloxacin, Levofloxacin and Moxifloxacin respectively. The proposed method is environmentally safe as it use water as a solvent. In addition, the procedure is simple and does not involve solvent extraction. The analytical performance of the method was fully validated, and the results were satisfactory.
Part IV: Application of surface Plasmon resonance of citrate capped silver nanoparticles for the selective determination of somefluoroquinolone drugs
This part describes simple and rapid colorimetric method for determination of (Ciprofloxacin, Ofloxacin, Levofloxacin, Moxifloxacin, Lomefloxacin and Pefloxacin( drugs using citrate-capped silver nanoparticles (Ag NPs). The absorption spectra and solution color of citrate-capped Ag NPs undergo dramatic changes upon exposure to the studied drugs with new surface Plasmon resonance (SPR) peaks appearing in the range of 598 – 696 nm, in addition to concomitant color change from yellow to blue. This is assumed to result from the aggregation of citrate-capped Ag NPs induced by the studied fluoroquinolones. Under the optimized conditions, good linear relationships (R2 = 0.9973–0.9991) were obtained in the range of 1.50–10.25 , 2.0–10.25, 2.0–10.50, 2.0–10.50, 1.5–10.0 and 2.0–11.0 µg/ml for Ciprofloxacin, Ofloxacin, Levofloxacin, Moxifloxacin, Lomefloxacin and Pefloxacin, respectively. The analytical performance of the method was fully validated, and the results were satisfactory. The developed method was successfully applied for the determination of the cited drugs in different pharmaceutical dosage forms with high degree of accuracy and precision. The proposed method is environmentally safe as it use water as a solvent. In addition, the procedure is simple and carried out without solvent extraction.